Chapter title |
Silver Filler Pre-embedding to Enhance Resolution and Contrast in Multidimensional SEM: A Nanoscale Imaging Study on Liver Tissue
|
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Chapter number | 33 |
Book title |
Nanoscale Imaging
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8591-3_33 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8590-6, 978-1-4939-8591-3
|
Authors |
Gerald J. Shami, Delfine Cheng, Filip Braet, Shami, Gerald J., Cheng, Delfine, Braet, Filip |
Abstract |
Contemporarily, serial block-face scanning electron microscopy (SBF-SEM) has emerged as an immensely powerful nanoscopic imaging technique, capable of generating large-volume three-dimensional information on a variety of biological specimens in a semiautomated manner. Despite the plethora of insights and advantages provided by SBF-SEM, a major challenge inherent to the technique is that of electron charging, which ultimately reduces attainable resolution and detracts from overall image quality. In this chapter, we describe a pre-embedding approach that involves infiltration of tissue with a highly conductive silver filler suspension following primary fixation. Such an approach is demonstrated to improve overall sample conductivity, resulting in the minimization of charging under high-vacuum conditions and an improvement in lateral resolution and image contrast. The strength of this sample preparation approach for SBF-SEM is illustrated on liver tissue. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 33% |
Professor | 1 | 17% |
Student > Postgraduate | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Unknown | 2 | 33% |