Chapter title |
Time-Resolved Imaging of Bacterial Surfaces Using Atomic Force Microscopy
|
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Chapter number | 23 |
Book title |
Nanoscale Imaging
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8591-3_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8590-6, 978-1-4939-8591-3
|
Authors |
Haig Alexander Eskandarian, Adrian Pascal Nievergelt, Georg Ernest Fantner, Eskandarian, Haig Alexander, Nievergelt, Adrian Pascal, Fantner, Georg Ernest |
Abstract |
Time-resolved atomic force microscopy (AFM) offers countless new modes by which to study bacterial cell physiology on relevant time scales, from mere milliseconds to hours and days on end. In addition, time-lapse AFM acts as a complementary tool to optical fluorescence microscopy (OFM), for which the combination offers a correlative link between the physical manifestation of bacterial phenotypes and molecular mechanisms obeying those principles. Herein we describe the essential materials and methods necessary for conducting time-resolved AFM and dual AFM/OFM experiments on bacteria. |
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