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Mendeley readers
| Chapter title |
High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates
|
|---|---|
| Chapter number | 7 |
| Book title |
Molecular Motors
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8556-2_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8554-8, 978-1-4939-8556-2
|
| Authors |
Keith J. Mickolajczyk, William O. Hancock, Mickolajczyk, Keith J., Hancock, William O. |
| Abstract |
This chapter describes methods for high-speed, unloaded, in vitro single-molecule kinesin tracking experiments. Instructions are presented for constructing a total internal reflection dark-field microscope (TIRDFM) and labeling motors with gold nanoparticles. An AMP-PNP unlocking assay is introduced as a specialized means of capturing processive events in a reduced field of view. Finally, step-finding tools for analyzing high frame-rate tracking data are described. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 30% |
| Researcher | 3 | 30% |
| Student > Bachelor | 2 | 20% |
| Professor | 1 | 10% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 30% |
| Biochemistry, Genetics and Molecular Biology | 2 | 20% |
| Physics and Astronomy | 2 | 20% |
| Chemistry | 1 | 10% |
| Engineering | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 1 | 10% |