| Chapter title |
In Vitro Maturation and Culture of Human Oocytes
|
|---|---|
| Chapter number | 3 |
| Book title |
Mouse Oocyte Development
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8603-3_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8602-6, 978-1-4939-8603-3
|
| Authors |
Jennifer R. Gruhn, Stine Gry Kristensen, Claus Yding Andersen, Eva R. Hoffmann, Gruhn, Jennifer R., Kristensen, Stine Gry, Andersen, Claus Yding, Hoffmann, Eva R. |
| Abstract |
We describe the collection, culture, and ex vivo, in vitro maturation of germinal vesicle (GV) oocytes obtained from human small antral follicles (hSAFs). hSAFs contain fully grown GV oocytes and have the advantages that they are more numerous than large or mature follicles, which are used in IVF treatment. hSAFs can be obtained directly from human ovarian tissue without exogenous gonadotrophin stimulation and therefore allows studies of oocytes even from young women and girls. The method described here was developed to study human female meiosis but could in theory also be used for fertility treatment. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 16% |
| Professor | 2 | 11% |
| Unspecified | 2 | 11% |
| Student > Postgraduate | 2 | 11% |
| Student > Bachelor | 1 | 5% |
| Other | 4 | 21% |
| Unknown | 5 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 21% |
| Medicine and Dentistry | 3 | 16% |
| Unspecified | 2 | 11% |
| Agricultural and Biological Sciences | 1 | 5% |
| Engineering | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 8 | 42% |