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Chapter title |
Monitoring Microtubule Dynamics in the Mouse Egg Using Photoactivatable-GFP-Tubulin
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Chapter number | 14 |
Book title |
Mouse Oocyte Development
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-8603-3_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8602-6, 978-1-4939-8603-3
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Authors |
Greg FitzHarris, FitzHarris, Greg |
Abstract |
Fluorescence photoactivation provides a strategy for monitoring protein kinetics within living cells. In particular, fluorescence photoactivation of a subpopulation of microtubule subunits within the spindle using photoactivatable fluorescent tubulin constructs has proven useful for assessing a variety of features of spindle microtubule dynamics, including poleward microtubule movement, microtubule depolymerization, and microtubule turnover, in various cellular settings. The current chapter describes a method for monitoring microtubule dynamics within the mouse egg spindle by photoactivation of photoactivatable-GFP-tubulin, followed by time-lapse confocal imaging. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 2 | 29% |
Researcher | 1 | 14% |
Unspecified | 1 | 14% |
Unknown | 3 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 29% |
Unspecified | 1 | 14% |
Design | 1 | 14% |
Unknown | 3 | 43% |