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Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics

Overview of attention for article published in Anais da Academia Brasileira de Ciencias, April 2018
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Title
Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics
Published in
Anais da Academia Brasileira de Ciencias, April 2018
DOI 10.1590/0001-3765201820170842
Pubmed ID
Authors

GLAUCIA B. CABRAL, VERA T.C. CARNEIRO, ANA CRISTINA M.M. GOMES, ANA LUIZA LACERDA, ADRIANA P. MARTINELLI, DIVA M.A. DUSI

Abstract

Brachiaria brizantha is a forage grass well adapted to tropical areas and cultivated in millions of hectares in Brazil. The apomictic mode of reproduction in this species, in addition to differences in ploidy between sexual and apomictic plants, impairs crossbreeding. The development of a methodology to transform apomictic cultivars will provide an option to introduce agronomic important traits to B. brizantha cv. Marandu. In addition, it will open the possibility to study in vivo the function of candidate genes involved in the apomictic reproduction. The objective of this work was to evaluate peeled seeds, isolated embryo from mature seeds, embryogenic calluses and embryogenic cell suspensions, as target explant for genetic transformation via biolistics. Plasmids bearing the marker genes gus and hptII under the control of the rice actin 1 promoter (pAct1-Os) or the maize ubiquitin 1 promoter (pUbi1Zm) were used. All the target-explants used were suitable for transient gene expression after bombardment, showing gus expression and resistance to hygromycin. Using embryogenic calluses and cell suspensions as target tissues, transgenic plants were regenerated and transgenes detected.

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Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 14%
Student > Doctoral Student 2 9%
Student > Bachelor 2 9%
Student > Ph. D. Student 2 9%
Professor 1 5%
Other 2 9%
Unknown 10 45%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 36%
Biochemistry, Genetics and Molecular Biology 1 5%
Unspecified 1 5%
Sports and Recreations 1 5%
Design 1 5%
Other 0 0%
Unknown 10 45%