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Plant Cell Culture Protocols

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Cover of 'Plant Cell Culture Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 An Introduction to Plant Tissue Culture: Advances and Perspectives
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    Chapter 2 Micropropagation in the Twenty-First Century
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    Chapter 3 Cellular and Morpho-histological Foundations of In Vitro Plant Regeneration
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    Chapter 4 Bacterial Endophytes in Plant Tissue Culture: Mode of Action, Detection, and Control
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    Chapter 5 Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture
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    Chapter 6 Selection of Molecular Markers for the Estimation of Somaclonal Variation
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    Chapter 7 Plant Tissue Culture: A Battle Horse in the Genome Editing Using CRISPR/Cas9
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    Chapter 8 Micropropagation of Agave Species
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    Chapter 9 Protocol for the Micropropagation of Coconut from Plumule Explants
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    Chapter 10 Micropropagation of Yucca Species
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    Chapter 11 Auxin Immunolocalization in Coffea canephora Tissues
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    Chapter 12 Somatic Embryogenesis in Common BeanPhaseolus vulgaris L.
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    Chapter 13 Induction of Somatic Embryogenesis in Jatropha curcas
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    Chapter 14 In Vitro Proliferation of Female Buds for Induction of Somatic Embryogenesis from False Horn Plantain (AAB, cv. Curraré)
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    Chapter 15 Somatic Embryogenesis in Theobroma cacao L.
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    Chapter 16 Somatic Embryogenesis of Quercus suber L. From Immature Zygotic Embryos
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    Chapter 17 Cryotherapy: A Novel Method for Virus Eradication in Economically Important Plant Species
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    Chapter 18 Cryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Technique
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    Chapter 19 Cryopreservation of Pollen Grains of Pineapple and Other Bromeliads
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    Chapter 20 Application of in Casa Pollination and Embryo Rescue Techniques for Breeding of Agave Species
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    Chapter 21 Haploid and Doubled Haploid Plant Production in Carrot Using Induced Parthenogenesis and Ovule Excision In Vitro
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    Chapter 22 Using Flow Cytometry Analysis in Plant Tissue Culture Derived Plants
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    Chapter 23 Procedure for Estimating the Tolerance and Accumulation of Heavy Metals Using Plant Cell Cultures
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    Chapter 24 Proteomics as a Tool to Study Molecular Changes During Plant Morphogenesis In Vitro
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    Chapter 25 Proteomic Analysis of Non-model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis, and MALDI Mass Spectrometry
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    Chapter 26 Chromatin Immunoprecipitation (ChiP) Protocol for the Analysis of Gene Regulation by Histone Modifications in Agave angustifolia Haw
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    Chapter 27 Transcription Factors: Their Role in the Regulation of Somatic Embryogenesis in Theobroma cacao L. and Other Species
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    Chapter 28 MicroRNA Expression and Regulation During Maize Somatic Embryogenesis
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    Chapter 29 Elaboration of Transcriptome During the Induction of Somatic Embryogenesis
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    Chapter 30 Induction of Specialized Metabolism in In Vitro Cultures of Capsicum chinense Jacq
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    Chapter 31 Analysis of Terpenoid Indole Alkaloids, Carotenoids, Phytosterols, and NMR-Based Metabolomics for Catharanthus roseus Cell Suspension Cultures
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    Chapter 32 Transformed Root Culture: From Genetic Transformation to NMR-Based Metabolomics
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    Chapter 33 Genetic Transformation of Pentalinon andrieuxii Tissue Cultures
Attention for Chapter 19: Cryopreservation of Pollen Grains of Pineapple and Other Bromeliads
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Chapter title
Cryopreservation of Pollen Grains of Pineapple and Other Bromeliads
Chapter number 19
Book title
Plant Cell Culture Protocols
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8594-4_19
Pubmed ID
Book ISBNs
978-1-4939-8593-7, 978-1-4939-8594-4
Authors

Fernanda Vidigal Duarte Souza, Everton Hilo de Souza, Ronilze Leite da Silva, Souza, Fernanda Vidigal Duarte, Souza, Everton Hilo de, Silva, Ronilze Leite da

Abstract

Cryopreservation of pollen grains is an efficient technique to overcome asynchronous flowering and to support actions for genetic improvement and conservation of important alleles. It can be used both by germplasm curators and plant breeders. In the case of Bromeliaceae, a family with wide diversity but also high vulnerability, the form of conservation can be crucial to prevent the increasing problem of genetic erosion. This chapter describes a method of cryopreservation of pollen grains of different Bromeliaceae species, including pineapple, after dehydration with silica and subsequent immersion in liquid nitrogen. The efficiency of this protocol has been demonstrated by the high pollen viability percentage and production of seeds after in vivo pollination with cryopreserved grains. The protocol can be used for cryopreserving pollen of many species of bromeliads and is easy to perform.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 9%
Student > Bachelor 1 9%
Professor 1 9%
Student > Ph. D. Student 1 9%
Researcher 1 9%
Other 2 18%
Unknown 4 36%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 36%
Biochemistry, Genetics and Molecular Biology 1 9%
Physics and Astronomy 1 9%
Engineering 1 9%
Unknown 4 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 July 2018.
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#20,525,274
of 23,094,276 outputs
Outputs from Methods in molecular biology
#9,976
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Outputs of similar age
#378,496
of 442,658 outputs
Outputs of similar age from Methods in molecular biology
#1,194
of 1,499 outputs
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So far Altmetric has tracked 13,207 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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