| Chapter title |
Plant Tissue Culture: A Battle Horse in the Genome Editing Using CRISPR/Cas9
|
|---|---|
| Chapter number | 7 |
| Book title |
Plant Cell Culture Protocols
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8594-4_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8593-7, 978-1-4939-8594-4
|
| Authors |
Víctor M. Loyola-Vargas, Randy N. Avilez-Montalvo, Loyola-Vargas, Víctor M., Avilez-Montalvo, Randy N. |
| Abstract |
Plant tissue culture (PTC) is a set of techniques for culturing cells, tissues, or organs in an aseptic medium with a defined chemical composition, in a controlled environment. Tissue culture, when combined with molecular biology techniques, becomes a powerful tool for the study of metabolic pathways, elucidation of cellular processes, genetic improvement and, through genetic engineering, the generation of cell lines resistant to biotic and abiotic stress, obtaining improved plants of agronomic interest, or studying the complex cellular genome. In this chapter, we analyze in general the use of plant tissue culture, in particular protoplasts and calli, in the implementation of CRISPR/Cas9 technology. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 21% |
| Student > Master | 6 | 21% |
| Student > Ph. D. Student | 5 | 18% |
| Student > Bachelor | 3 | 11% |
| Professor > Associate Professor | 2 | 7% |
| Other | 4 | 14% |
| Unknown | 2 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 61% |
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| Business, Management and Accounting | 1 | 4% |
| Unspecified | 1 | 4% |
| Chemistry | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 2 | 7% |