Chapter title |
Cryotherapy: A Novel Method for Virus Eradication in Economically Important Plant Species
|
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Chapter number | 17 |
Book title |
Plant Cell Culture Protocols
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8594-4_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8593-7, 978-1-4939-8594-4
|
Authors |
Min-Rui Wang, Long Chen, Zhibo Zhang, Dag-Ragnar Blystad, Qiao-Chun Wang, Wang, Min-Rui, Chen, Long, Zhang, Zhibo, Blystad, Dag-Ragnar, Wang, Qiao-Chun |
Abstract |
Virus diseases have been a great threat to production of economically important crops. In practice, the use of virus-free planting material is an effective strategy to control viral diseases. Cryotherapy, developed based on cryopreservation, is a novel plant biotechnology tool for virus eradication. Comparing to the traditional meristem culture for virus elimination, cryotherapy resulted in high efficiency of pathogen eradication. In general, cryotherapy includes seven major steps: (1) introduction of infected plant materials into in vitro cultures, (2) shoot tip excision, (3) tolerance induction of explants to dehydration and subsequent freezing in liquid nitrogen (LN), (4) a short-time treatment of explants in LN, (5) warming and post-culture for regeneration, (6) re-establishment of regenerated plants in greenhouse conditions, and (7) virus indexing. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 19% |
Student > Bachelor | 2 | 8% |
Student > Master | 2 | 8% |
Professor > Associate Professor | 2 | 8% |
Professor | 1 | 4% |
Other | 2 | 8% |
Unknown | 12 | 46% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 38% |
Biochemistry, Genetics and Molecular Biology | 2 | 8% |
Chemistry | 1 | 4% |
Unknown | 13 | 50% |