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Plant Cell Culture Protocols

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Cover of 'Plant Cell Culture Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 An Introduction to Plant Tissue Culture: Advances and Perspectives
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    Chapter 2 Micropropagation in the Twenty-First Century
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    Chapter 3 Cellular and Morpho-histological Foundations of In Vitro Plant Regeneration
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    Chapter 4 Bacterial Endophytes in Plant Tissue Culture: Mode of Action, Detection, and Control
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    Chapter 5 Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture
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    Chapter 6 Selection of Molecular Markers for the Estimation of Somaclonal Variation
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    Chapter 7 Plant Tissue Culture: A Battle Horse in the Genome Editing Using CRISPR/Cas9
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    Chapter 8 Micropropagation of Agave Species
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    Chapter 9 Protocol for the Micropropagation of Coconut from Plumule Explants
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    Chapter 10 Micropropagation of Yucca Species
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    Chapter 11 Auxin Immunolocalization in Coffea canephora Tissues
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    Chapter 12 Somatic Embryogenesis in Common BeanPhaseolus vulgaris L.
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    Chapter 13 Induction of Somatic Embryogenesis in Jatropha curcas
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    Chapter 14 In Vitro Proliferation of Female Buds for Induction of Somatic Embryogenesis from False Horn Plantain (AAB, cv. Curraré)
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    Chapter 15 Somatic Embryogenesis in Theobroma cacao L.
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    Chapter 16 Somatic Embryogenesis of Quercus suber L. From Immature Zygotic Embryos
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    Chapter 17 Cryotherapy: A Novel Method for Virus Eradication in Economically Important Plant Species
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    Chapter 18 Cryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Technique
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    Chapter 19 Cryopreservation of Pollen Grains of Pineapple and Other Bromeliads
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    Chapter 20 Application of in Casa Pollination and Embryo Rescue Techniques for Breeding of Agave Species
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    Chapter 21 Haploid and Doubled Haploid Plant Production in Carrot Using Induced Parthenogenesis and Ovule Excision In Vitro
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    Chapter 22 Using Flow Cytometry Analysis in Plant Tissue Culture Derived Plants
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    Chapter 23 Procedure for Estimating the Tolerance and Accumulation of Heavy Metals Using Plant Cell Cultures
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    Chapter 24 Proteomics as a Tool to Study Molecular Changes During Plant Morphogenesis In Vitro
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    Chapter 25 Proteomic Analysis of Non-model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis, and MALDI Mass Spectrometry
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    Chapter 26 Chromatin Immunoprecipitation (ChiP) Protocol for the Analysis of Gene Regulation by Histone Modifications in Agave angustifolia Haw
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    Chapter 27 Transcription Factors: Their Role in the Regulation of Somatic Embryogenesis in Theobroma cacao L. and Other Species
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    Chapter 28 MicroRNA Expression and Regulation During Maize Somatic Embryogenesis
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    Chapter 29 Elaboration of Transcriptome During the Induction of Somatic Embryogenesis
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    Chapter 30 Induction of Specialized Metabolism in In Vitro Cultures of Capsicum chinense Jacq
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    Chapter 31 Analysis of Terpenoid Indole Alkaloids, Carotenoids, Phytosterols, and NMR-Based Metabolomics for Catharanthus roseus Cell Suspension Cultures
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    Chapter 32 Transformed Root Culture: From Genetic Transformation to NMR-Based Metabolomics
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    Chapter 33 Genetic Transformation of Pentalinon andrieuxii Tissue Cultures
Attention for Chapter 2: Micropropagation in the Twenty-First Century
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Citations

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Chapter title
Micropropagation in the Twenty-First Century
Chapter number 2
Book title
Plant Cell Culture Protocols
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8594-4_2
Pubmed ID
Book ISBNs
978-1-4939-8593-7, 978-1-4939-8594-4
Authors

Jean Carlos Cardoso, Lee Tseng Sheng Gerald, Jaime A. Teixeira da Silva, Cardoso, Jean Carlos, Sheng Gerald, Lee Tseng, Teixeira da Silva, Jaime A.

Abstract

Despite more than a century of research on effective biotechnological methods, micropropagation continues to be an important tool for the large-scale production of clonal plantlets of several important plant species that retain genetic fidelity and are pest-free. In some cases, micropropagation is the only technique that supports the maintenance and promotes the economic value of specific agricultural species. The micropropagation of plants solved many phytosanitary problems and allowed both the expansion and access to high-quality plants for growers from different countries and economic backgrounds, thereby effectively contributing to an agricultural expansion in this and the last century. The challenges for micropropagation in the twenty-first century include cost reduction, enhanced efficiency, developing new technologies, and combining micropropagation with other systems/propagation techniques such as microcuttings, hydroponics, and aeroponics. In this chapter, we discuss the actual uses of micropropagation in this century, its importance and limitations, and some possible techniques that can effectively increase its wider application by replacing certain conventional techniques and technologies.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 142 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 142 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 21 15%
Student > Master 17 12%
Researcher 13 9%
Student > Ph. D. Student 10 7%
Other 4 3%
Other 15 11%
Unknown 62 44%
Readers by discipline Count As %
Agricultural and Biological Sciences 46 32%
Biochemistry, Genetics and Molecular Biology 21 15%
Environmental Science 2 1%
Chemistry 2 1%
Chemical Engineering 1 <1%
Other 3 2%
Unknown 67 47%