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Two nucleotide second messengers regulate the production of the Vibrio cholerae colonization factor GbpA

Overview of attention for article published in BMC Microbiology, August 2015
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  • High Attention Score compared to outputs of the same age and source (81st percentile)

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3 tweeters

Citations

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13 Dimensions

Readers on

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24 Mendeley
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Title
Two nucleotide second messengers regulate the production of the Vibrio cholerae colonization factor GbpA
Published in
BMC Microbiology, August 2015
DOI 10.1186/s12866-015-0506-5
Pubmed ID
Authors

Ankunda T. Kariisa, Alyssa Grube, Rita Tamayo

Abstract

The nucleotide second messengers cAMP and c-di-GMP allow many bacteria, including the human intestinal pathogen Vibrio cholerae, to respond to environmental stimuli with appropriate physiological adaptations. In response to limitation of specific carbohydrates, cAMP and its receptor CRP control the transcription of genes important for nutrient acquisition and utilization; c-di-GMP controls the transition between motile and sessile lifestyles often, but not exclusively, through transcriptional mechanisms. In this study, we investigated the convergence of cAMP and c-di-GMP signaling pathways in regulating the expression of gbpA. GbpA is a colonization factor that participates in the attachment of V. cholerae to N-acetylglucosamine-containing surfaces in its native aquatic environment and the host intestinal tract. We show that c-di-GMP inhibits gbpA activation in a fashion independent of the known transcription factors that directly sense c-di-GMP. Interestingly, inhibition of gbpA activation by c-di-GMP only occurs during growth on non-PTS dependent nutrient sources. Consistent with this result, we show that CRP binds to the gbpA promoter in a cAMP-dependent manner in vitro and drives transcription of gbpA in vivo. The interplay between cAMP and c-di-GMP does not broadly impact the CRP-cAMP regulon, but occurs more specifically at the gbpA promoter. These findings suggest that c-di-GMP directly interferes with the interaction of CRP-cAMP and the gbpA promoter via an unidentified regulator. The use of two distinct second messenger signaling mechanisms to regulate gbpA transcription may allow V. cholerae to finely modulate GbpA production, and therefore colonization of aquatic and host surfaces, in response to discrete environmental stimuli.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 4%
Unknown 23 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 38%
Researcher 4 17%
Student > Bachelor 2 8%
Student > Master 2 8%
Professor > Associate Professor 2 8%
Other 5 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 29%
Biochemistry, Genetics and Molecular Biology 7 29%
Unspecified 3 13%
Immunology and Microbiology 3 13%
Psychology 1 4%
Other 3 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 March 2016.
All research outputs
#2,780,883
of 7,424,901 outputs
Outputs from BMC Microbiology
#359
of 1,259 outputs
Outputs of similar age
#70,015
of 227,767 outputs
Outputs of similar age from BMC Microbiology
#11
of 59 outputs
Altmetric has tracked 7,424,901 research outputs across all sources so far. This one has received more attention than most of these and is in the 62nd percentile.
So far Altmetric has tracked 1,259 research outputs from this source. They receive a mean Attention Score of 2.9. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 227,767 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 59 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.