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Attention Score in Context
| Title |
Effects of freezing storage on the DNA extraction and microbial evaluation from anaerobic digested sludges
|
|---|---|
| Published in |
BMC Research Notes, September 2015
|
| DOI | 10.1186/s13104-015-1407-2 |
| Pubmed ID | |
| Authors |
Valeria Romanazzi, Deborah Traversi, Eugenio Lorenzi, Giorgio Gilli |
| Abstract |
The anaerobic digestion is one of the most spread renewable energy technology. The input biomasses included various environmental problematic wastes such as sludge coming from wastewater treatment plant (WWTP) and organic fraction of municipal solid waste (OFMSW). As biomolecular procedures have become important tools for the microbial characterisation of anaerobic samples coming from the reactors, it is crucial sampling and extracting properly DNA in order to employ such types of techniques. The current study is aimed to evaluate how freezing temperature and length of storage at -20 °C influence both the extracted DNA yield and microbial community quantifications from digested sludge samples collected at full-scale plants. From WWTP sludge samples, we observed a reduction of DNA concentration comparing fresh and stored samples for 10 days at -20 °C (ANOVA test p < 0.0001), with an estimated DNA loss of approximately 65 % for such types of samples, however the methanogen communities can be assessed respecting the fresh conditions. From OFMSW sludge samples, we observed a reduction in extracted DNA (-90 %), after 120 frozen days, while microbial communities are determined respecting the fresh conditions within 2 months of frozen storage. The remarkable effect of frozen storage on sludge samples suggests as the better procedure to perform the DNA extraction from fresh sample. On the other hand it is not generally possible, so approximately 2 months of storage at -20 °C appears to be suitable time at which DNA concentrations remain sufficient to perform coherent microbial characterization through quantitative qRT-PCR. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 33% |
| Australia | 1 | 17% |
| Unknown | 3 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 50% |
| Members of the public | 3 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 64 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 20% |
| Student > Bachelor | 13 | 20% |
| Student > Master | 11 | 17% |
| Student > Doctoral Student | 4 | 6% |
| Researcher | 4 | 6% |
| Other | 6 | 9% |
| Unknown | 13 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Environmental Science | 10 | 16% |
| Biochemistry, Genetics and Molecular Biology | 10 | 16% |
| Agricultural and Biological Sciences | 8 | 13% |
| Engineering | 7 | 11% |
| Medicine and Dentistry | 3 | 5% |
| Other | 8 | 13% |
| Unknown | 18 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 June 2016.
All research outputs
#14,441,291
of 25,452,734 outputs
Outputs from BMC Research Notes
#1,674
of 4,516 outputs
Outputs of similar age
#125,944
of 279,165 outputs
Outputs of similar age from BMC Research Notes
#62
of 173 outputs
Altmetric has tracked 25,452,734 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,516 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one has gotten more attention than average, scoring higher than 62% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,165 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 173 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.