Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin.
Journal of applied oral science : revista FOB, July 2018
Andrucioli, Marcela Cristina Damião, Matsumoto, Mírian Aiko Nakane, Saraiva, Maria Conceição Pereira, Feres, Magda, Figueiredo, Luciene Cristina de, Sorgi, Carlos Artério, Faccioli, Lucia Helena, Silva, Raquel Assed Bezerra da, Silva, Lea Assed Bezerra da, Nelson-Filho, Paulo
Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay. The 15 successful and 10 failed mini-implants (1.6 mm diameter × 7.0 or 9.0 mm long), placed in the maxilla and/or mandible, were obtained from 15 patients undergoing orthodontic treatment. Data were analyzed statistically by the Wilcoxon rank-sum test using the SAS software (a=0.05). All 40 microbial species were detected in both groups of mini-implants, with different frequencies. No differences were observed between the groups with respect to microbial complexes (blue, purple, yellow, green, orange, red and other species) and endotoxin quantification (p>0.05). Neither microbial contamination nor endotoxin quantification was determinant for the early loss of stability of the mini-implants.
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