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Xenopus

Overview of attention for book
Cover of 'Xenopus'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Husbandry, General Care, and Transportation of Xenopus laevis and Xenopus tropicalis
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    Chapter 2 Generation and Care of Xenopus laevis and Xenopus tropicalis Embryos
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    Chapter 3 Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering
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    Chapter 4 Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology
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    Chapter 5 Genotyping of CRISPR/Cas9 Genome Edited Xenopus tropicalis
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    Chapter 6 BATCH-GE: Analysis of NGS Data for Genome Editing Assessment
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    Chapter 7 A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair
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    Chapter 8 How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique
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    Chapter 9 Targeted Electroporation in the CNS in Xenopus Embryos
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    Chapter 10 Conditional Chemogenetic Ablation of Photoreceptor Cells in Xenopus Retina
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    Chapter 11 Cancer Models in Xenopus tropicalis by CRISPR/Cas9 Mediated Knockout of Tumor Suppressors
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    Chapter 12 CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis
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    Chapter 13 Quantitative Proteomics of Xenopus Embryos I, Sample Preparation
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    Chapter 14 Quantitative Proteomics for Xenopus Embryos II, Data Analysis
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    Chapter 15 Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous System
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    Chapter 16 X-FaCT: Xenopus-Fast Clearing Technique
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    Chapter 17 Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry
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    Chapter 18 Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary Epidermis
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    Chapter 19 Evaluating Blood Cell Populations in Xenopus Using Flow Cytometry and Differential Counts by Cytospin
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    Chapter 20 Isolation and Culture of Amphibian (Xenopus laevis) Sub-Capsular Liver and Bone Marrow Cells
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    Chapter 21 Isolation and Primary Culture Methods of Adult and Larval Myogenic Cells from Xenopus laevis
Attention for Chapter 15: Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous System
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Chapter title
Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous System
Chapter number 15
Book title
Xenopus
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8784-9_15
Pubmed ID
Book ISBNs
978-1-4939-8783-2, 978-1-4939-8784-9
Authors

Weiss, Lukas, Offner, Thomas, Hassenklöver, Thomas, Manzini, Ivan, Lukas Weiss, Thomas Offner, Thomas Hassenklöver, Ivan Manzini

Abstract

Electroporation is an efficient method of transferring charged macromolecules into living cells in order to study their morphology, function, and connectivity within neuronal networks. Labeling cells with fluorophore-coupled macromolecules can be used to trace projections of whole neuronal ensembles, as well as the fine morphology of single cells. Here, we present a protocol to visualize pre- and postsynaptic components of a sensory relay synapse in the brain, using the olfactory system of Xenopus laevis tadpoles as a model. We apply bulk electroporation to trace projections of receptor neurons from the nose to the brain, and single cell electroporation to visualize the morphology of their synaptic target cells, the mitral-tufted cells. Labeling the receptor neurons with a calcium-sensitive dye allows us to record stimulus-induced presynaptic input to the dendrites of the postsynaptic cells via functional calcium imaging.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 22%
Student > Master 2 22%
Professor 2 22%
Student > Bachelor 1 11%
Student > Doctoral Student 1 11%
Other 0 0%
Unknown 1 11%
Readers by discipline Count As %
Neuroscience 4 44%
Biochemistry, Genetics and Molecular Biology 2 22%
Agricultural and Biological Sciences 1 11%
Pharmacology, Toxicology and Pharmaceutical Science 1 11%
Unknown 1 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 September 2018.
All research outputs
#10,270,581
of 13,459,952 outputs
Outputs from Methods in molecular biology
#3,769
of 8,488 outputs
Outputs of similar age
#184,283
of 266,299 outputs
Outputs of similar age from Methods in molecular biology
#1
of 1 outputs
Altmetric has tracked 13,459,952 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,488 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 266,299 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them