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Xenopus

Overview of attention for book
Cover of 'Xenopus'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Husbandry, General Care, and Transportation of Xenopus laevis and Xenopus tropicalis
  3. Altmetric Badge
    Chapter 2 Generation and Care of Xenopus laevis and Xenopus tropicalis Embryos
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    Chapter 3 Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering
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    Chapter 4 Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology
  6. Altmetric Badge
    Chapter 5 Genotyping of CRISPR/Cas9 Genome Edited Xenopus tropicalis
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    Chapter 6 BATCH-GE: Analysis of NGS Data for Genome Editing Assessment
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    Chapter 7 A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair
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    Chapter 8 How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique
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    Chapter 9 Targeted Electroporation in the CNS in Xenopus Embryos
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    Chapter 10 Conditional Chemogenetic Ablation of Photoreceptor Cells in Xenopus Retina
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    Chapter 11 Cancer Models in Xenopus tropicalis by CRISPR/Cas9 Mediated Knockout of Tumor Suppressors
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    Chapter 12 CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis
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    Chapter 13 Quantitative Proteomics of Xenopus Embryos I, Sample Preparation
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    Chapter 14 Quantitative Proteomics for Xenopus Embryos II, Data Analysis
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    Chapter 15 Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous System
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    Chapter 16 X-FaCT: Xenopus-Fast Clearing Technique
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    Chapter 17 Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry
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    Chapter 18 Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary Epidermis
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    Chapter 19 Evaluating Blood Cell Populations in Xenopus Using Flow Cytometry and Differential Counts by Cytospin
  21. Altmetric Badge
    Chapter 20 Isolation and Culture of Amphibian (Xenopus laevis) Sub-Capsular Liver and Bone Marrow Cells
  22. Altmetric Badge
    Chapter 21 Isolation and Primary Culture Methods of Adult and Larval Myogenic Cells from Xenopus laevis
Attention for Chapter 3: Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering
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Mentioned by

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3 tweeters

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4 Dimensions

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18 Mendeley
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Chapter title
Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering
Chapter number 3
Book title
Xenopus
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8784-9_3
Pubmed ID
Book ISBNs
978-1-4939-8783-2, 978-1-4939-8784-9
Authors

Thomas Naert, Kris Vleminckx, Naert, Thomas, Vleminckx, Kris

Abstract

In this chapter, we convey a state-of-the art update to the 2014 Nakayama protocol for CRISPR/Cas9 genome engineering in Xenopus tropicalis (X. tropicalis). We discuss in depth, gRNA design software and rules, gRNA synthesis, and procedures for tissue- and tissue-specific CRISPR/Cas9 genome editing by targeted microinjection in X. tropicalis embryos. We demonstrate the methodology by which any standard equipped Xenopus researcher with microinjection experience can generate F0 CRISPR/Cas9 mediated mosaic mutants (crispants) within one to two work-week(s). The described methodology allows CRISPR/Cas9 efficiencies to be high enough to read out phenotypic consequences, and thus perform gene function analysis, in the F0 crispant. Additionally, we provide the framework for performing multiplex tissue-specific CRISPR/Cas9 experiments generating crispants mosaic mutant in up to four genes simultaneously, which can be of importance for Laevis researchers aiming to target by CRISPR/Cas9 both the S and L homeolog of a gene simultaneously. Finally, we discuss off-target concerns, how to minimize these and ways to rapidly bypass reviewer off-target critique by exploiting the advantages of X. tropicalis.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 39%
Professor > Associate Professor 3 17%
Student > Master 3 17%
Professor 1 6%
Student > Bachelor 1 6%
Other 2 11%
Unknown 1 6%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 39%
Agricultural and Biological Sciences 5 28%
Chemical Engineering 1 6%
Immunology and Microbiology 1 6%
Medicine and Dentistry 1 6%
Other 1 6%
Unknown 2 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 April 2019.
All research outputs
#9,164,513
of 14,568,570 outputs
Outputs from Methods in molecular biology
#3,013
of 8,763 outputs
Outputs of similar age
#163,359
of 272,500 outputs
Outputs of similar age from Methods in molecular biology
#1
of 1 outputs
Altmetric has tracked 14,568,570 research outputs across all sources so far. This one is in the 24th percentile – i.e., 24% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,763 research outputs from this source. They receive a mean Attention Score of 2.5. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 272,500 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them