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Xenopus

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Cover of 'Xenopus'

Table of Contents

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    Book Overview
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    Chapter 1 Husbandry, General Care, and Transportation of Xenopus laevis and Xenopus tropicalis
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    Chapter 2 Generation and Care of Xenopus laevis and Xenopus tropicalis Embryos
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    Chapter 3 Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering
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    Chapter 4 Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology
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    Chapter 5 Genotyping of CRISPR/Cas9 Genome Edited Xenopus tropicalis
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    Chapter 6 BATCH-GE: Analysis of NGS Data for Genome Editing Assessment
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    Chapter 7 A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair
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    Chapter 8 How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique
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    Chapter 9 Targeted Electroporation in the CNS in Xenopus Embryos
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    Chapter 10 Conditional Chemogenetic Ablation of Photoreceptor Cells in Xenopus Retina
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    Chapter 11 Cancer Models in Xenopus tropicalis by CRISPR/Cas9 Mediated Knockout of Tumor Suppressors
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    Chapter 12 CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis
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    Chapter 13 Quantitative Proteomics of Xenopus Embryos I, Sample Preparation
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    Chapter 14 Quantitative Proteomics for Xenopus Embryos II, Data Analysis
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    Chapter 15 Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous System
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    Chapter 16 X-FaCT: Xenopus-Fast Clearing Technique
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    Chapter 17 Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry
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    Chapter 18 Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary Epidermis
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    Chapter 19 Evaluating Blood Cell Populations in Xenopus Using Flow Cytometry and Differential Counts by Cytospin
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    Chapter 20 Isolation and Culture of Amphibian (Xenopus laevis) Sub-Capsular Liver and Bone Marrow Cells
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    Chapter 21 Isolation and Primary Culture Methods of Adult and Larval Myogenic Cells from Xenopus laevis
Attention for Chapter 7: A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair
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Chapter title
A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair
Chapter number 7
Book title
Xenopus
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8784-9_7
Pubmed ID
Book ISBNs
978-1-4939-8783-2, 978-1-4939-8784-9
Authors

Ken-ich T. Suzuki, Yuto Sakane, Miyuki Suzuki, Takashi Yamamoto

Abstract

Following completion of the genome sequences of Xenopus tropicalis and X. laevis, gene targeting techniques have become increasingly important for the further development of Xenopus research in the life sciences. Gene knockout using programmable nucleases, such as TALEN and CRISPR/Cas9, has reached a level whereby we can readily and routinely perform loss-of-function analysis of genes of interest in these species. However, there is still room for improvement in gene knock-in techniques owing to some technical problems. To overcome these problems, several knock-in techniques have been developed. Among them, we introduce in this chapter a simple knock-in system mediated by microhomology mediated end joining repair. This protocol allows us to produce knock-in animals for in vivo tagging, promoter/enhancer traps, and transgenesis in both of these Xenopus species.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 4 33%
Student > Master 2 17%
Student > Ph. D. Student 1 8%
Researcher 1 8%
Professor 1 8%
Other 0 0%
Unknown 3 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 33%
Biochemistry, Genetics and Molecular Biology 2 17%
Immunology and Microbiology 1 8%
Medicine and Dentistry 1 8%
Unknown 4 33%