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Absence of association between Plasmodium falciparum small sub-unit ribosomal RNA gene mutations and in vitro decreased susceptibility to doxycycline

Overview of attention for article published in Malaria Journal, September 2015
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3 tweeters

Citations

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3 Dimensions

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19 Mendeley
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Title
Absence of association between Plasmodium falciparum small sub-unit ribosomal RNA gene mutations and in vitro decreased susceptibility to doxycycline
Published in
Malaria Journal, September 2015
DOI 10.1186/s12936-015-0878-x
Pubmed ID
Authors

Tiphaine Gaillard, Nathalie Wurtz, Sandrine Houzé, Kanlaya Sriprawat, Chirapat Wangsing, Véronique Hubert, Jacques Lebras, François Nosten, Sébastien Briolant, Bruno Pradines

Abstract

Doxycycline is an antibiotic used in combination with quinine or artesunate for malaria treatment or alone for malaria chemoprophylaxis. Recently, one prophylactic failure has been reported, and several studies have highlighted in vitro doxycycline decreased susceptibility in Plasmodium falciparum isolates from different areas. The genetic markers that contribute to detecting and monitoring the susceptibility of P. falciparum to doxycycline, the pfmdt and pftetQ genes, have recently been identified. However, these markers are not sufficient to explain in vitro decreased susceptibility of P. falciparum to doxycycline. In this paper, the association between polymorphism of the small sub-unit ribosomal RNA apicoplastic gene pfssrRNA (PFC10_API0057) and in vitro susceptibilities of P. falciparum isolates to doxycycline were investigated. Doxycycline IC50 determinations using the hypoxanthine uptake inhibition assay were performed on 178 African and Thai P. falciparum isolates. The polymorphism of pfssrRNA was investigated in these samples by standard PCR followed by sequencing. No point mutations were found in pfssrRNA in the Thai or African isolates, regardless of the determined IC50 values. The pfssrRNA gene is not associated with in vitro decreased susceptibility of P. falciparum to doxycycline. Identifying new in vitro molecular markers associated with reduced susceptibility is needed, to survey the emergence of doxycycline resistance.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 5%
Unknown 18 95%

Demographic breakdown

Readers by professional status Count As %
Other 5 26%
Student > Bachelor 4 21%
Researcher 3 16%
Student > Doctoral Student 2 11%
Student > Master 2 11%
Other 2 11%
Unknown 1 5%
Readers by discipline Count As %
Medicine and Dentistry 10 53%
Pharmacology, Toxicology and Pharmaceutical Science 5 26%
Agricultural and Biological Sciences 2 11%
Mathematics 1 5%
Unknown 1 5%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 September 2015.
All research outputs
#9,612,425
of 15,683,828 outputs
Outputs from Malaria Journal
#3,281
of 4,443 outputs
Outputs of similar age
#125,337
of 248,229 outputs
Outputs of similar age from Malaria Journal
#1
of 1 outputs
Altmetric has tracked 15,683,828 research outputs across all sources so far. This one is in the 36th percentile – i.e., 36% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,443 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 248,229 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them