Title |
Effects of drying and storage conditions on the stability of TSH in blood spots
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Published in |
Archives of Endocrinology and Metabolism, March 2018
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DOI | 10.20945/2359-3997000000026 |
Pubmed ID | |
Authors |
Patrícia Künzle Ribeiro Magalhães, Carlos Henrique Miranda, Fernando Crivelenti Vilar, André Schmidt, Roberta Rodrigues Bittar, Giselle Aparecida Caixe de Carvalho Paixão, Edson Zangiacomi Martinez, Léa Maria Zanini Maciel |
Abstract |
To evaluate the influence of sample drying and storage temperature on TSH stability in neonatal screening. Blood samples from 29 adult volunteers as a surrogate for neonatal blood (10 with normal TSH, 9 with overt hypothyroid and 10 with subclinical hypothyroidism) were spotted on filter paper and dried at 22°C or 35°C for 3 hours. The samples were then stored at 22°C, -4°C, or -20°C, and TSH measurements were performed at day 0 (D0), D7, D30, D60, D180, and D360 of storage. The drying temperature did not interfere with TSH measurement on D0. TSH values remained stable up to D30 when stored at 22°C and were stable up to D60 when stored in a refrigerator or freezer. Samples stored at 22°C had a greater decrease in TSH values than samples stored in a refrigerator or a freezer. Freezer storage is not advantageous compared to storage in the refrigerator. At the end of one year, if confirmation of the initial result is required, a reduction of TSH concentrations should be taken into account. |
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Unknown | 9 | 41% |