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Reverse-transcription, loop-mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses

Overview of attention for article published in Virology Journal, September 2015
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Title
Reverse-transcription, loop-mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses
Published in
Virology Journal, September 2015
DOI 10.1186/s12985-015-0378-1
Pubmed ID
Authors

Sisi Luo, Zhixun Xie, Liji Xie, Jiabo Liu, Zhiqin Xie, Xianwen Deng, Li Huang, Jiaoling Huang, Tingting Zeng, Mazhar I. Khan

Abstract

The H10 subtype avian influenza viruses (H10N4, H10N5 and H10N7) have been reported to cause disease in mammals, and the first human case of H10N8 subtype avian influenza virus was reported in 2013. Recently, H10 subtype avian influenza viruses (AIVs) have been followed more closely, but routine diagnostic tests are tedious, less sensitive and time consuming, rapid molecular detection assays for H10 AIVs are not available. Based on conserved sequences within the HA gene of the H10 subtype AIVs, specific primer sets of H10 subtype of AIVs were designed and assay reaction conditions were optimized. A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was established for the rapid detection of H10 subtype AIVs. The specificity was validated using multiple subtypes of AIVs and other avian respiratory pathogens, and the limit of detection (LOD) was tested using concentration gradient of in vitro-transcribed RNA. The established assay was performed in a water bath at 63 °C for 40 min, and the amplification result was visualized directly as well as under daylight reflections. The H10-RT-LAMP assay can specifically amplify H10 subtype AIVs and has no cross-reactivity with other subtypes AIVs or avian pathogens. The LOD of the H10-RT-LAMP assay was 10 copies per μL of in vitro-transcribed RNA. The RT-LAMP method reported here is demonstrated to be a potentially valuable means for the detection of H10 subtype AIV and rapid clinical diagnosis, being fast, simple, and low in cost. Consequently, it will be a very useful screening assay for the surveillance of H10 subtype AIVs in underequipped laboratories as well as in field conditions.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 40%
Professor 2 13%
Student > Doctoral Student 2 13%
Student > Ph. D. Student 2 13%
Student > Master 1 7%
Other 2 13%
Readers by discipline Count As %
Medicine and Dentistry 3 20%
Agricultural and Biological Sciences 2 13%
Unspecified 2 13%
Biochemistry, Genetics and Molecular Biology 2 13%
Veterinary Science and Veterinary Medicine 2 13%
Other 4 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2015.
All research outputs
#4,706,091
of 6,368,969 outputs
Outputs from Virology Journal
#1,135
of 1,455 outputs
Outputs of similar age
#137,121
of 199,280 outputs
Outputs of similar age from Virology Journal
#52
of 61 outputs
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