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Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum

Overview of attention for article published in Nature Communications, January 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (96th percentile)
  • Good Attention Score compared to outputs of the same age and source (70th percentile)

Mentioned by

news
5 news outlets
blogs
1 blog
twitter
2 tweeters
facebook
1 Facebook page

Citations

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20 Dimensions

Readers on

mendeley
52 Mendeley
Title
Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum
Published in
Nature Communications, January 2015
DOI 10.1038/ncomms9410
Pubmed ID
Authors

Sangwoo Kim, Yu-Sin Jang, Sung-Chul Ha, Jae-Woo Ahn, Eun-Jung Kim, Jae Hong Lim, Changhee Cho, Yong Shin Ryu, Sung Kuk Lee, Sang Yup Lee, Kyung-Jin Kim

Abstract

Thiolase is the first enzyme catalysing the condensation of two acetyl-coenzyme A (CoA) molecules to form acetoacetyl-CoA in a dedicated pathway towards the biosynthesis of n-butanol, an important solvent and biofuel. Here we elucidate the crystal structure of Clostridium acetobutylicum thiolase (CaTHL) in its reduced/oxidized states. CaTHL, unlike those from other aerobic bacteria such as Escherichia coli and Zoogloea ramegera, is regulated by the redox-switch modulation through reversible disulfide bond formation between two catalytic cysteine residues, Cys88 and Cys378. When CaTHL is overexpressed in wild-type C. acetobutylicum, butanol production is reduced due to the disturbance of acidogenic to solventogenic shift. The CaTHL(V77Q/N153Y/A286K) mutant, which is not able to form disulfide bonds, exhibits higher activity than wild-type CaTHL, and enhances butanol production upon overexpression. On the basis of these results, we suggest that CaTHL functions as a key enzyme in the regulation of the main metabolism of C. acetobutylicum through a redox-switch regulatory mechanism.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 1 2%
China 1 2%
United States 1 2%
Netherlands 1 2%
New Zealand 1 2%
Unknown 47 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 21 40%
Researcher 8 15%
Student > Postgraduate 5 10%
Student > Master 5 10%
Unspecified 4 8%
Other 9 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 37%
Biochemistry, Genetics and Molecular Biology 17 33%
Unspecified 5 10%
Chemical Engineering 2 4%
Engineering 2 4%
Other 7 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 47. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2016.
All research outputs
#149,722
of 7,084,078 outputs
Outputs from Nature Communications
#2,514
of 10,770 outputs
Outputs of similar age
#9,016
of 231,963 outputs
Outputs of similar age from Nature Communications
#208
of 701 outputs
Altmetric has tracked 7,084,078 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,770 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 40.1. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 231,963 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 701 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.