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Herbicide-resistant cotton (Gossypium hirsutum) plants: an alternative way of manual weed removal

Overview of attention for article published in BMC Research Notes, September 2015
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Title
Herbicide-resistant cotton (Gossypium hirsutum) plants: an alternative way of manual weed removal
Published in
BMC Research Notes, September 2015
DOI 10.1186/s13104-015-1397-0
Pubmed ID
Authors

Ayesha Latif, Abdul Qayyum Rao, Muhammad Azmat Ullah Khan, Naila Shahid, Kamran Shehzad Bajwa, Muhammad Aleem Ashraf, Malik Adil Abbas, Muhammad Azam, Ahmad Ali Shahid, Idrees Ahmad Nasir, Tayyab Husnain

Abstract

Cotton yield has been badly affected by different insects and weed competition. In Past Application of multiple chemicals is required to manage insects and weed control was achieved by different conventional means, such as hand weeding, crop rotation and polyculture, because no synthetic chemicals were available. The control methods shifted towards high input and target-oriented methods after the discovery of synthetic herbicide in the 1930s. To utilise the transgenic approach, cotton plants expressing the codon-optimised CEMB GTGene were produced in the present study. Local cotton variety CEMB-02 containing Cry1Ac and Cry2A in single cassette was transformed by synthetic codon-optimised 5-enolpyruvylshikimate-3-phosphate synthase gene cloned into pCAMBIA 1301 vector under 35S promoter with Agrobacterium tumifaciens. Putative transgenic plants were screened in MS medium containing 120 µmol/L glyphosate. Integration and expression of the gene were evaluated by PCR from genomic DNA and ELISA from protein. A 1.4-kb PCR product for Glyphosate and 167-bp product for Cry2A were obtained by amplification through gene specific primers. Expression level of Glyphosate and Bt proteins in two transgenic lines were recorded to be 0.362, 0.325 µg/g leaf and 0.390, 0.300 µg/g leaf respectively. FISH analysis of transgenic lines demonstrates the presence of one and two copy no. of Cp4 EPSPS transgene respectively. Efficacy of the transgene Cp4 EPSPS was further evaluated by Glyphosate spray (41 %) assay at 1900 ml/acre and insect bioassay which shows 100 %mortality of insect feeding on transgenic lines as compared to control. The present study shows that the transgenic lines produced in this study were resistant not only to insects but also equally good against 1900 ml/acre field spray concentration of glyphosate.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 7%
Unknown 14 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 27%
Student > Master 3 20%
Researcher 2 13%
Other 2 13%
Lecturer > Senior Lecturer 1 7%
Other 3 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 53%
Biochemistry, Genetics and Molecular Biology 3 20%
Unspecified 2 13%
Environmental Science 1 7%
Veterinary Science and Veterinary Medicine 1 7%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 September 2015.
All research outputs
#11,135,220
of 12,519,627 outputs
Outputs from BMC Research Notes
#2,345
of 2,804 outputs
Outputs of similar age
#202,997
of 246,176 outputs
Outputs of similar age from BMC Research Notes
#6
of 9 outputs
Altmetric has tracked 12,519,627 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,804 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.