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Plant Transcription Factors

Overview of attention for book
Cover of 'Plant Transcription Factors'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective
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    Chapter 2 Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants
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    Chapter 3 The Long-Term “In Natura” Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments
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    Chapter 4 Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs
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    Chapter 5 A Specific Knockdown of Transcription Factor Activities in Arabidopsis
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    Chapter 6 Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
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    Chapter 7 CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha
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    Chapter 8 Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines
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    Chapter 9 Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells
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    Chapter 10 Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation
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    Chapter 11 High Impact Gene Discovery: Simple Strand-Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network
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    Chapter 12 Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti
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    Chapter 13 NanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing
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    Chapter 14 Genome-Wide TSS Identification in Maize
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    Chapter 15 Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee
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    Chapter 16 Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci
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    Chapter 17 Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines
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    Chapter 18 Rapid and Quantitative CELD Assay to Measure the Specificity of Transcription Factor-DNA-Binding Interactions and Identify cis-Elements
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    Chapter 19 In Situ Proximity Ligation Assay to Detect the Interaction Between Plant Transcription Factors and Other Regulatory Proteins
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    Chapter 20 Cell-Free Protein Synthesis of Plant Transcription Factors
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    Chapter 21 Application of MNase-Seq in the Global Mapping of Nucleosome Positioning in Plants
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    Chapter 22 Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato
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    Chapter 23 Genome-Wide Identification of Chromatin Domains Anchored at the Nuclear Periphery in Plants
Attention for Chapter 22: Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato
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Chapter title
Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato
Chapter number 22
Book title
Plant Transcription Factors
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8657-6_22
Pubmed ID
Book ISBNs
978-1-4939-8656-9, 978-1-4939-8657-6
Authors

Ren Li, Xia Cui, Li, Ren, Cui, Xia

Abstract

DNase I hypersensitive sites (DHSs) are genomic regions that exhibit hypersensitivity to DNase I cleavage. DHSs appear to be an essential feature of "open chromatin" structure in eukaryotes. Most of regulatory elements and the majority of transcription factor-binding sites are associated with open chromatin marked by DHSs. DNase I digestion followed by high-throughput DNA sequencing (DNase-seq) has become a powerful tool to reveal chromatin status and identify regulatory elements at genome-wide level. Here, we developed a DNase-seq procedure in tomato revised from previous methods in other plants, involving plant nuclei isolation, digestion of purified nuclei with DNase I, collection of DNase-trimmed DNA fragments, validation of DHS sample quality by qPCR, and DNase-seq library preparation. We introduced an AMPure XP beads system for the selection of the desirable DNA fragment. DHS datasets will provide the dynamic profiles of regulatory elements during plant development.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
South Africa 1 11%
Unknown 8 89%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 33%
Student > Ph. D. Student 1 11%
Student > Bachelor 1 11%
Other 1 11%
Unknown 3 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 33%
Agricultural and Biological Sciences 3 33%
Unknown 3 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 July 2018.
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#20,527,576
of 23,096,849 outputs
Outputs from Methods in molecular biology
#9,977
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#378,510
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Outputs of similar age from Methods in molecular biology
#1,194
of 1,499 outputs
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