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Plant Transcription Factors

Overview of attention for book
Cover of 'Plant Transcription Factors'

Table of Contents

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    Book Overview
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    Chapter 1 Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective
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    Chapter 2 Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants
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    Chapter 3 The Long-Term “In Natura” Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments
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    Chapter 4 Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs
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    Chapter 5 A Specific Knockdown of Transcription Factor Activities in Arabidopsis
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    Chapter 6 Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
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    Chapter 7 CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha
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    Chapter 8 Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines
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    Chapter 9 Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells
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    Chapter 10 Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation
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    Chapter 11 High Impact Gene Discovery: Simple Strand-Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network
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    Chapter 12 Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti
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    Chapter 13 NanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing
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    Chapter 14 Genome-Wide TSS Identification in Maize
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    Chapter 15 Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee
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    Chapter 16 Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci
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    Chapter 17 Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines
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    Chapter 18 Rapid and Quantitative CELD Assay to Measure the Specificity of Transcription Factor-DNA-Binding Interactions and Identify cis-Elements
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    Chapter 19 In Situ Proximity Ligation Assay to Detect the Interaction Between Plant Transcription Factors and Other Regulatory Proteins
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    Chapter 20 Cell-Free Protein Synthesis of Plant Transcription Factors
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    Chapter 21 Application of MNase-Seq in the Global Mapping of Nucleosome Positioning in Plants
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    Chapter 22 Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato
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    Chapter 23 Genome-Wide Identification of Chromatin Domains Anchored at the Nuclear Periphery in Plants
Attention for Chapter 15: Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee
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Chapter title
Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee
Chapter number 15
Book title
Plant Transcription Factors
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8657-6_15
Pubmed ID
Book ISBNs
978-1-4939-8656-9, 978-1-4939-8657-6

Yoko Mizuta, Katsutoshi Tsuda


In plants, transcription factors often act as cell-to-cell trafficking mobile proteins and specify cell fate. Thus, to visualize spatiotemporal expression pattern and localization of transcription factors are essential to understand their functions during development. Several protocols have been developed to observe fluorescent protein. However, plant-specific autofluorescent compounds and various tissue components with different refractive indexes interfere with detection of fluorescent signals of your interest. Furthermore, cell fate specification often occurs in a limited number of cells covered by lateral/layers of organs. To overcome those issues, the plant clearing method, ClearSee, was recently developed for high-resolution imaging inside tissues by making background transparent. In this chapter, we provide three-dimensional imaging of fluorescent-protein-fused transcription factors by two-photon excitation microscopy in Arabidopsis and rice. Complex cell patterning with gene expression could be observed from any direction three-dimensionally. This method could be applicable to visualize any protein of your interest or it can readily be adapted in various other plants.

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Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 50%
Researcher 1 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 50%
Neuroscience 1 50%

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