Chapter title |
Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells
|
---|---|
Chapter number | 9 |
Book title |
Plant Transcription Factors
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8657-6_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8656-9, 978-1-4939-8657-6
|
Authors |
Kyohei Uemoto, Takashi Araki, Motomu Endo, Uemoto, Kyohei, Araki, Takashi, Endo, Motomu |
Abstract |
Cell-type-specific transcription factors are key to deducing the distinct functions of specialized cells from gene expression profiles. Mesophyll is a major tissue for photosynthesis, and contributes about 80% of total RNA from leaves. Palisade and spongy mesophyll cells are sub-tissues that have different morphologies and physiologies. Thus, determining the palisade and spongy mesophyll-specific transcription factors from the respective sub-tissue-specific transcriptomes is vital to understanding or verifying functions of major plant tissues. One way in which gene expression profiles can be addressed is through direct isolation. Here, we present rapid and simple methods to isolate palisade and spongy mesophyll cells mechanically and enzymatically. This method provides a good yield of each isolated cell type, and the isolated cells can be used for various downstream applications. |
Mendeley readers
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Country | Count | As % |
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Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 20% |
Student > Master | 3 | 20% |
Researcher | 2 | 13% |
Student > Bachelor | 1 | 7% |
Unknown | 6 | 40% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 47% |
Biochemistry, Genetics and Molecular Biology | 1 | 7% |
Environmental Science | 1 | 7% |
Unknown | 6 | 40% |