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A rapid assay for Hendra virus IgG antibody detection and its titre estimation using magnetic nanoparticles and phycoerythrin

Overview of attention for article published in Journal of Virological Methods, September 2015
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2 Facebook pages

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25 Mendeley
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Title
A rapid assay for Hendra virus IgG antibody detection and its titre estimation using magnetic nanoparticles and phycoerythrin
Published in
Journal of Virological Methods, September 2015
DOI 10.1016/j.jviromet.2015.05.008
Pubmed ID
Authors

Yuan Gao, Jackie Pallister, Florian Lapierre, Gary Crameri, Lin-Fa Wang, Yonggang Zhu

Abstract

Detection of Hendra viral IgG antibody in animal sera is useful for surveillance following a virus outbreak. The commonly used enzyme-linked immunosorbent assay and fluorescence-based Luminex assay typically consist of three steps and take at least several hours to complete. We have simplified the procedure to two steps in an effort to develop a rapid procedure for IgG antibody, but not IgM antibody, detection. This is achieved by conjugating the fluorescence label R-phycoerythrin directly onto the IgG binding protein Protein G. The use of magnetic nanoparticles, due to their large specific surface area, has helped reduce each of the binding steps to 20min. As a result, the whole assay can be completed in 60min. We also demonstrate a method to quickly determine IgG antibody titres by assaying the sera at only two dilutions (i.e. 1:20 and 1:1000) and using the fluorescence. The results of this approach correlated well with the well-regarded serum neutralization test in virus antibody assays. This protocol reported here can be adopted in Luminex assays, fluorescence-linked immunosorbent assays and assays on microfluidics platforms for rapid antibody surveillance of Hendra and other viruses.

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 24%
Student > Master 5 20%
Researcher 3 12%
Student > Bachelor 3 12%
Professor 2 8%
Other 4 16%
Unknown 2 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 36%
Biochemistry, Genetics and Molecular Biology 4 16%
Medicine and Dentistry 2 8%
Immunology and Microbiology 1 4%
Neuroscience 1 4%
Other 3 12%
Unknown 5 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2015.
All research outputs
#9,814,275
of 12,286,894 outputs
Outputs from Journal of Virological Methods
#1,815
of 2,141 outputs
Outputs of similar age
#172,515
of 250,762 outputs
Outputs of similar age from Journal of Virological Methods
#18
of 32 outputs
Altmetric has tracked 12,286,894 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,141 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 250,762 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.