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Vibrio Cholerae

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Cover of 'Vibrio Cholerae'

Table of Contents

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    Book Overview
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    Chapter 1 Laboratory Culturing Techniques and Maintenance of Vibrio cholerae
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    Chapter 2 Genotypic and Phenotypic Assays to Distinguish Vibrio cholerae Biotype
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    Chapter 3 Preparation of Vibrio cholerae Samples for RNA-seq Analysis
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    Chapter 4 Random Transposon Mutagenesis of Vibrio cholerae
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    Chapter 5 Metabolomics of Vibrio cholerae
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    Chapter 6 Natural Cotransformation and Multiplex Genome Editing by Natural Transformation (MuGENT) of Vibrio cholerae
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    Chapter 7 Chromatin Immunoprecipitation
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    Chapter 8 Fly Models of Vibrio cholerae Infection and Colonization
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    Chapter 9 Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholerae
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    Chapter 10 Transposon Sequencing of Vibrio cholerae in the Infant Rabbit Model of Cholera
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    Chapter 11 Isolation of Outer Membrane Vesicles Including Their Quantitative and Qualitative Analyses
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    Chapter 12 Utilization of Vibrio cholerae as a Model Organism to Screen Natural Product Libraries for Identification of New Antibiotics
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    Chapter 13 Infant Mouse Model of Vibrio cholerae Infection and Colonization
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    Chapter 14 Methods for Assessments of Collagenolytic Activity of the Vibrio cholerae Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin
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    Chapter 15 Proteomics of Vibrio cholerae
Attention for Chapter 10: Transposon Sequencing of Vibrio cholerae in the Infant Rabbit Model of Cholera
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Chapter title
Transposon Sequencing of Vibrio cholerae in the Infant Rabbit Model of Cholera
Chapter number 10
Book title
Vibrio Cholerae
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8685-9_10
Pubmed ID
Book ISBNs
978-1-4939-8684-2, 978-1-4939-8685-9
Authors

Lauren M. Shull, Andrew Camilli, Shull, Lauren M., Camilli, Andrew

Abstract

Transposon sequencing, or Tn-seq, combines transposon mutagenesis and massively parallel sequencing to allow for rapid and high-throughput identification of genes that play roles in fitness within environments of interest. The bacterial pathogen Vibrio cholerae is an excellent candidate for Tn-seq screens due to the availability of a plasmid-based in vivo transposition system and the relative ease with which the cholera disease state can be modeled in animals. This chapter will describe a method for performing Tn-seq screens on V. cholerae in the infant rabbit model of cholera.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 21%
Student > Ph. D. Student 3 21%
Student > Master 1 7%
Researcher 1 7%
Professor > Associate Professor 1 7%
Other 1 7%
Unknown 4 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 29%
Immunology and Microbiology 2 14%
Agricultural and Biological Sciences 2 14%
Business, Management and Accounting 1 7%
Unknown 5 36%