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Vibrio Cholerae

Overview of attention for book
Cover of 'Vibrio Cholerae'

Table of Contents

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    Book Overview
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    Chapter 1 Laboratory Culturing Techniques and Maintenance of Vibrio cholerae
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    Chapter 2 Genotypic and Phenotypic Assays to Distinguish Vibrio cholerae Biotype
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    Chapter 3 Preparation of Vibrio cholerae Samples for RNA-seq Analysis
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    Chapter 4 Random Transposon Mutagenesis of Vibrio cholerae
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    Chapter 5 Metabolomics of Vibrio cholerae
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    Chapter 6 Natural Cotransformation and Multiplex Genome Editing by Natural Transformation (MuGENT) of Vibrio cholerae
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    Chapter 7 Chromatin Immunoprecipitation
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    Chapter 8 Fly Models of Vibrio cholerae Infection and Colonization
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    Chapter 9 Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholerae
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    Chapter 10 Transposon Sequencing of Vibrio cholerae in the Infant Rabbit Model of Cholera
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    Chapter 11 Isolation of Outer Membrane Vesicles Including Their Quantitative and Qualitative Analyses
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    Chapter 12 Utilization of Vibrio cholerae as a Model Organism to Screen Natural Product Libraries for Identification of New Antibiotics
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    Chapter 13 Infant Mouse Model of Vibrio cholerae Infection and Colonization
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    Chapter 14 Methods for Assessments of Collagenolytic Activity of the Vibrio cholerae Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin
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    Chapter 15 Proteomics of Vibrio cholerae
Attention for Chapter 12: Utilization of Vibrio cholerae as a Model Organism to Screen Natural Product Libraries for Identification of New Antibiotics
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Chapter title
Utilization of Vibrio cholerae as a Model Organism to Screen Natural Product Libraries for Identification of New Antibiotics
Chapter number 12
Book title
Vibrio Cholerae
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8685-9_12
Pubmed ID
Book ISBNs
978-1-4939-8684-2, 978-1-4939-8685-9
Authors

Aleksandra E. Sikora, Richard Tehan, Kerry McPhail

Abstract

The development of antibiotic-resistant bacteria requires increasing research efforts in drug discovery. Vibrio cholerae can be utilized as a model gram-negative enteric pathogen in high- and medium-throughput screening campaigns to identify antimicrobials with different modes of action. In this chapter, we describe methods for the optimal growth of V. cholerae in 384-well plates, preparation of suitable microtiter natural product sample libraries, as well as their screening using measurements of bacterial density and activity of type II secretion-dependent protease as readouts. Concomitant LC-MS/MS profiling and spectral data networking of assay sample libraries facilitate dereplication of putative known and/or nuisance compounds and efficient prioritization of samples containing putative new natural products for further investigation.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 33%
Student > Bachelor 1 33%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Immunology and Microbiology 1 33%
Unknown 1 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 July 2018.
All research outputs
#11,809,618
of 13,309,886 outputs
Outputs from Methods in molecular biology
#5,989
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Outputs of similar age
#232,438
of 268,514 outputs
Outputs of similar age from Methods in molecular biology
#1
of 1 outputs
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