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Chapter title |
Preparation of Vibrio cholerae Samples for RNA-seq Analysis
|
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Chapter number | 3 |
Book title |
Vibrio Cholerae
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-8685-9_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8684-2, 978-1-4939-8685-9
|
Authors |
Jyl S. Matson, Matson, Jyl S. |
Abstract |
Massively parallel cDNA sequencing (RNA-seq) is a powerful tool for providing an unbiased approach to assess transcript abundance under a variety of conditions. In comparison to microarrays, this technique provides increased resolution and sensitivity and the ability to identify rare transcripts and sRNAs. Here, we describe the sample preparation (based on Illumina technology) used for transcriptomic analysis of V. cholerae cDNA libraries. We describe the entire process from RNA isolation through to the generation of barcoded cDNA libraries ready for sequencing. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 100% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 1 | 100% |