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Vibrio Cholerae

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Cover of 'Vibrio Cholerae'

Table of Contents

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    Book Overview
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    Chapter 1 Laboratory Culturing Techniques and Maintenance of Vibrio cholerae
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    Chapter 2 Genotypic and Phenotypic Assays to Distinguish Vibrio cholerae Biotype
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    Chapter 3 Preparation of Vibrio cholerae Samples for RNA-seq Analysis
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    Chapter 4 Random Transposon Mutagenesis of Vibrio cholerae
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    Chapter 5 Metabolomics of Vibrio cholerae
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    Chapter 6 Natural Cotransformation and Multiplex Genome Editing by Natural Transformation (MuGENT) of Vibrio cholerae
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    Chapter 7 Chromatin Immunoprecipitation
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    Chapter 8 Fly Models of Vibrio cholerae Infection and Colonization
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    Chapter 9 Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholerae
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    Chapter 10 Transposon Sequencing of Vibrio cholerae in the Infant Rabbit Model of Cholera
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    Chapter 11 Isolation of Outer Membrane Vesicles Including Their Quantitative and Qualitative Analyses
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    Chapter 12 Utilization of Vibrio cholerae as a Model Organism to Screen Natural Product Libraries for Identification of New Antibiotics
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    Chapter 13 Infant Mouse Model of Vibrio cholerae Infection and Colonization
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    Chapter 14 Methods for Assessments of Collagenolytic Activity of the Vibrio cholerae Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin
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    Chapter 15 Proteomics of Vibrio cholerae
Attention for Chapter 7: Chromatin Immunoprecipitation
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Citations

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Chapter title
Chromatin Immunoprecipitation
Chapter number 7
Book title
Vibrio Cholerae
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8685-9_7
Pubmed ID
Book ISBNs
978-1-4939-8684-2, 978-1-4939-8685-9
Authors

Julio C. Ayala, Jorge A. Benitez, Anisia J. Silva

Abstract

Chromatin immunoprecipitation (ChIP) measures the physical association between a protein and DNA in the cell. In combination with next-generation sequencing, the technique enables the identification of DNA targets for the corresponding protein across an entire genome. Here we describe the immunoprecipitation of Vibrio cholerae DNA bound to the histone-like nucleoid structuring protein (H-NS) tagged with the Flag epitope. The quality of the DNA obtained in this protocol is suitable for next-generation sequencing. The procedure described herein can be readily adapted to other bacteria and DNA-binding proteins.

Mendeley readers

The data shown below were compiled from readership statistics for 104 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 2 2%
Denmark 1 <1%
Brazil 1 <1%
Unknown 100 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 29 28%
Researcher 23 22%
Student > Master 13 13%
Student > Bachelor 7 7%
Professor > Associate Professor 5 5%
Other 15 14%
Unknown 12 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 51 49%
Biochemistry, Genetics and Molecular Biology 29 28%
Medicine and Dentistry 4 4%
Engineering 2 2%
Earth and Planetary Sciences 1 <1%
Other 3 3%
Unknown 14 13%