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Vibrio Cholerae

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Cover of 'Vibrio Cholerae'

Table of Contents

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    Book Overview
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    Chapter 1 Laboratory Culturing Techniques and Maintenance of Vibrio cholerae
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    Chapter 2 Genotypic and Phenotypic Assays to Distinguish Vibrio cholerae Biotype
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    Chapter 3 Preparation of Vibrio cholerae Samples for RNA-seq Analysis
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    Chapter 4 Random Transposon Mutagenesis of Vibrio cholerae
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    Chapter 5 Metabolomics of Vibrio cholerae
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    Chapter 6 Natural Cotransformation and Multiplex Genome Editing by Natural Transformation (MuGENT) of Vibrio cholerae
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    Chapter 7 Chromatin Immunoprecipitation
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    Chapter 8 Fly Models of Vibrio cholerae Infection and Colonization
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    Chapter 9 Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholerae
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    Chapter 10 Transposon Sequencing of Vibrio cholerae in the Infant Rabbit Model of Cholera
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    Chapter 11 Isolation of Outer Membrane Vesicles Including Their Quantitative and Qualitative Analyses
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    Chapter 12 Utilization of Vibrio cholerae as a Model Organism to Screen Natural Product Libraries for Identification of New Antibiotics
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    Chapter 13 Infant Mouse Model of Vibrio cholerae Infection and Colonization
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    Chapter 14 Methods for Assessments of Collagenolytic Activity of the Vibrio cholerae Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin
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    Chapter 15 Proteomics of Vibrio cholerae
Attention for Chapter 14: Methods for Assessments of Collagenolytic Activity of the Vibrio cholerae Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin
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Chapter title
Methods for Assessments of Collagenolytic Activity of the Vibrio cholerae Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin
Chapter number 14
Book title
Vibrio Cholerae
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8685-9_14
Pubmed ID
Book ISBNs
978-1-4939-8684-2, 978-1-4939-8685-9
Authors

Bo R. Weber, Aleksandra E. Sikora

Abstract

Secreted proteases have been linked to facilitating the survival of Vibrio cholerae in different environmental niches. Examination of protease activity using various measures is critical to determine the substrate specificity of a given proteolytic enzyme and its biological function. Here, we describe methods to assess collagenolytic activity of the V. cholerae extracellular proteases using synthetic and natural substrates in quantitative and qualitative assays. Purification of the secreted V. cholerae collagenase VchC and extraction of type I collagen from fish skin are also described.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 1 25%
Student > Bachelor 1 25%
Student > Master 1 25%
Unknown 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 25%
Social Sciences 1 25%
Engineering 1 25%
Unknown 1 25%