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Vibrio Cholerae

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Cover of 'Vibrio Cholerae'

Table of Contents

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    Book Overview
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    Chapter 1 Laboratory Culturing Techniques and Maintenance of Vibrio cholerae
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    Chapter 2 Genotypic and Phenotypic Assays to Distinguish Vibrio cholerae Biotype
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    Chapter 3 Preparation of Vibrio cholerae Samples for RNA-seq Analysis
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    Chapter 4 Random Transposon Mutagenesis of Vibrio cholerae
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    Chapter 5 Metabolomics of Vibrio cholerae
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    Chapter 6 Natural Cotransformation and Multiplex Genome Editing by Natural Transformation (MuGENT) of Vibrio cholerae
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    Chapter 7 Chromatin Immunoprecipitation
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    Chapter 8 Fly Models of Vibrio cholerae Infection and Colonization
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    Chapter 9 Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholerae
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    Chapter 10 Transposon Sequencing of Vibrio cholerae in the Infant Rabbit Model of Cholera
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    Chapter 11 Isolation of Outer Membrane Vesicles Including Their Quantitative and Qualitative Analyses
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    Chapter 12 Utilization of Vibrio cholerae as a Model Organism to Screen Natural Product Libraries for Identification of New Antibiotics
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    Chapter 13 Infant Mouse Model of Vibrio cholerae Infection and Colonization
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    Chapter 14 Methods for Assessments of Collagenolytic Activity of the Vibrio cholerae Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin
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    Chapter 15 Proteomics of Vibrio cholerae
Attention for Chapter 4: Random Transposon Mutagenesis of Vibrio cholerae
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Chapter title
Random Transposon Mutagenesis of Vibrio cholerae
Chapter number 4
Book title
Vibrio Cholerae
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8685-9_4
Pubmed ID
Book ISBNs
978-1-4939-8684-2, 978-1-4939-8685-9
Authors

Jyl S. Matson

Abstract

Transposon-based random mutagenesis of bacterial genomes has proven to be a powerful genetic tool for the identification of genes and regulatory elements that contribute to specific phenotypes. One such approach that has been used in Vibrio cholerae for many years is the introduction of mariner transposons to generate random libraries of mutants. These libraries have been successfully used for a wide variety of genetic screens and selections in this important bacterial pathogen. Here we present a detailed protocol for the use of plasmid pFD1 (containing the mariner transposon magellan3) to create mutant libraries in V. cholerae.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 20%
Researcher 1 20%
Student > Master 1 20%
Unknown 2 40%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 40%
Unspecified 1 20%
Unknown 2 40%