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Mendeley readers
| Chapter title |
Random Transposon Mutagenesis of Vibrio cholerae
|
|---|---|
| Chapter number | 4 |
| Book title |
Vibrio Cholerae
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8685-9_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8684-2, 978-1-4939-8685-9
|
| Authors |
Jyl S. Matson, Matson, Jyl S. |
| Abstract |
Transposon-based random mutagenesis of bacterial genomes has proven to be a powerful genetic tool for the identification of genes and regulatory elements that contribute to specific phenotypes. One such approach that has been used in Vibrio cholerae for many years is the introduction of mariner transposons to generate random libraries of mutants. These libraries have been successfully used for a wide variety of genetic screens and selections in this important bacterial pathogen. Here we present a detailed protocol for the use of plasmid pFD1 (containing the mariner transposon magellan3) to create mutant libraries in V. cholerae. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 5 | 71% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Unknown | 5 | 71% |