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Histone Variants

Overview of attention for book
Cover of 'Histone Variants'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Methods for Preparing Nucleosomes Containing Histone Variants
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    Chapter 2 Characterization of Posttranslational Modifications on Histone Variants
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    Chapter 3 Purification of Histone Variant-Interacting Chaperone Complexes
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    Chapter 4 Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics
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    Chapter 5 Histone Native Chromatin Immunoprecipitation
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    Chapter 6 How to Tackle Challenging ChIP-Seq, with Long-Range Cross-Linking, Using ATRX as an Example
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    Chapter 7 time-ChIP: A Method to Determine Long-Term Locus-Specific Nucleosome Inheritance
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    Chapter 8 MINCE-Seq: Mapping In Vivo Nascent Chromatin with EdU and Sequencing
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    Chapter 9 RChIP-Seq: Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes
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    Chapter 10 Bioinformatic Analysis of Nucleosome and Histone Variant Positioning
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    Chapter 11 Imaging Newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Tag System
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    Chapter 12 Real-Time De Novo Deposition of Centromeric Histone-Associated Proteins Using the Auxin-Inducible Degradation System
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    Chapter 13 Live Imaging of Parental Histone Variant Dynamics in UVC-Damaged Chromatin
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    Chapter 14 CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A
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    Chapter 15 Studying the Evolution of Histone Variants Using Phylogeny
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    Chapter 16 Characterization of Post-Meiotic Male Germ Cell Genome Organizational States
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    Chapter 17 An Animal Model for Genetic Analysis of Multi-Gene Families: Cloning and Transgenesis of Large Tandemly Repeated Histone Gene Clusters
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    Chapter 18 Imaging and Quantitation of Assembly Dynamics of the Centromeric Histone H3 Variant CENP-A in Drosophila melanogaster Spermatocytes by Immunofluorescence and Fluorescence In-Situ Hybridization (Immuno-FISH)
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    Chapter 19 Probing the Function of Oncohistones Using Mutant Transgenes and Knock-In Mutations
Attention for Chapter 1: Methods for Preparing Nucleosomes Containing Histone Variants
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Chapter title
Methods for Preparing Nucleosomes Containing Histone Variants
Chapter number 1
Book title
Histone Variants
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8663-7_1
Pubmed ID
30073519
Book ISBNs
978-1-4939-8662-0, 978-1-4939-8663-7
Authors

Tomoya Kujirai, Yasuhiro Arimura, Risa Fujita, Naoki Horikoshi, Shinichi Machida, Hitoshi Kurumizaka

Abstract

Histone variants are key epigenetic players that regulate transcription, repair, replication, and recombination of genomic DNA. Histone variant incorporation into nucleosomes induces structural diversity of nucleosomes, consequently leading to the structural versatility of chromatin. Such chromatin diversity created by histone variants may play a central role in the epigenetic regulation of genes. Each histone variant possesses specific biochemical and physical characteristics, and thus the preparation methods are complicated. Here, we introduce the methods for the purification of human histone variants as recombinant proteins, and describe the preparation methods for histone complexes and nucleosomes containing various histone variants. We also describe the detailed method for the preparation of heterotypic nucleosomes, which may function in certain biological phenomena. These methods are useful for biochemical, structural, and biophysical studies.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 43 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 26%
Student > Ph. D. Student 7 16%
Student > Master 6 14%
Other 3 7%
Student > Bachelor 3 7%
Other 2 5%
Unknown 11 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 24 56%
Chemistry 4 9%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Medicine and Dentistry 1 2%
Agricultural and Biological Sciences 1 2%
Other 0 0%
Unknown 12 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 August 2018.
All research outputs
#18,645,475
of 23,098,660 outputs
Outputs from Methods in molecular biology
#7,989
of 13,208 outputs
Outputs of similar age
#254,520
of 331,034 outputs
Outputs of similar age from Methods in molecular biology
#133
of 193 outputs
Altmetric has tracked 23,098,660 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,208 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 193 others from the same source and published within six weeks on either side of this one. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.

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