↓ Skip to main content

Histone Variants

Overview of attention for book
Cover of 'Histone Variants'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Methods for Preparing Nucleosomes Containing Histone Variants
  3. Altmetric Badge
    Chapter 2 Characterization of Posttranslational Modifications on Histone Variants
  4. Altmetric Badge
    Chapter 3 Purification of Histone Variant-Interacting Chaperone Complexes
  5. Altmetric Badge
    Chapter 4 Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics
  6. Altmetric Badge
    Chapter 5 Histone Native Chromatin Immunoprecipitation
  7. Altmetric Badge
    Chapter 6 How to Tackle Challenging ChIP-Seq, with Long-Range Cross-Linking, Using ATRX as an Example
  8. Altmetric Badge
    Chapter 7 time-ChIP: A Method to Determine Long-Term Locus-Specific Nucleosome Inheritance
  9. Altmetric Badge
    Chapter 8 MINCE-Seq: Mapping In Vivo Nascent Chromatin with EdU and Sequencing
  10. Altmetric Badge
    Chapter 9 RChIP-Seq: Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes
  11. Altmetric Badge
    Chapter 10 Bioinformatic Analysis of Nucleosome and Histone Variant Positioning
  12. Altmetric Badge
    Chapter 11 Imaging Newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Tag System
  13. Altmetric Badge
    Chapter 12 Real-Time De Novo Deposition of Centromeric Histone-Associated Proteins Using the Auxin-Inducible Degradation System
  14. Altmetric Badge
    Chapter 13 Live Imaging of Parental Histone Variant Dynamics in UVC-Damaged Chromatin
  15. Altmetric Badge
    Chapter 14 CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A
  16. Altmetric Badge
    Chapter 15 Studying the Evolution of Histone Variants Using Phylogeny
  17. Altmetric Badge
    Chapter 16 Characterization of Post-Meiotic Male Germ Cell Genome Organizational States
  18. Altmetric Badge
    Chapter 17 An Animal Model for Genetic Analysis of Multi-Gene Families: Cloning and Transgenesis of Large Tandemly Repeated Histone Gene Clusters
  19. Altmetric Badge
    Chapter 18 Imaging and Quantitation of Assembly Dynamics of the Centromeric Histone H3 Variant CENP-A in Drosophila melanogaster Spermatocytes by Immunofluorescence and Fluorescence In-Situ Hybridization (Immuno-FISH)
  20. Altmetric Badge
    Chapter 19 Probing the Function of Oncohistones Using Mutant Transgenes and Knock-In Mutations
Attention for Chapter 12: Real-Time De Novo Deposition of Centromeric Histone-Associated Proteins Using the Auxin-Inducible Degradation System
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (67th percentile)
  • High Attention Score compared to outputs of the same age and source (89th percentile)

Mentioned by

twitter
9 X users

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
6 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Real-Time De Novo Deposition of Centromeric Histone-Associated Proteins Using the Auxin-Inducible Degradation System
Chapter number 12
Book title
Histone Variants
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8663-7_12
Pubmed ID
Book ISBNs
978-1-4939-8662-0, 978-1-4939-8663-7
Authors

Sebastian Hoffmann, Daniele Fachinetti, Hoffmann, Sebastian, Fachinetti, Daniele

Abstract

Measuring protein dynamics is essential to uncover protein function and to understand the formation of large protein complexes such as centromeres. Recently, genome engineering in human cells has improved our ability to study the function of endogenous proteins. By combining genome editing techniques with the auxin-inducible degradation (AID) system, we created a versatile tool to study protein dynamics. This system allows us to analyze both protein function and dynamics by enabling rapid protein depletion and reexpression in the same experimental setup. Here, we focus on the dynamics of the centromeric histone-associated protein CENP-C, responsible for the formation of the kinetochore complex. Following rapid removal and reactivation of a fluorescent version of CENP-C by auxin treatment and removal, we could follow CENP-C de novo deposition at centromeric regions during different stages of the cell cycle. In conclusion, the auxin degradation system is a powerful tool to assess and quantify protein dynamics in real time.

X Demographics

X Demographics

The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 50%
Professor 1 17%
Other 1 17%
Unknown 1 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 67%
Nursing and Health Professions 1 17%
Unknown 1 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2018.
All research outputs
#6,612,623
of 24,608,500 outputs
Outputs from Methods in molecular biology
#1,938
of 13,844 outputs
Outputs of similar age
#106,738
of 335,761 outputs
Outputs of similar age from Methods in molecular biology
#22
of 195 outputs
Altmetric has tracked 24,608,500 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 13,844 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 335,761 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 195 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.