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Mendeley readers
| Chapter title |
Histone Native Chromatin Immunoprecipitation
|
|---|---|
| Chapter number | 5 |
| Book title |
Histone Variants
|
| Published in |
Methods in molecular biology, August 2018
|
| DOI | 10.1007/978-1-4939-8663-7_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8662-0, 978-1-4939-8663-7
|
| Authors |
Alicia Alonso, Emily Bernstein, Dan Hasson |
| Abstract |
Chromatin immunoprecipitation (ChIP) is becoming the standard method to study genome-wide distribution of histone variants and histone posttranslational modifications (PTMs). In this chapter, we describe a detailed native ChIP protocol and downstream procedures for the preparation of DNA libraries for next-generation sequencing. Compared to cross-linked ChIP, "native" ChIP has been shown to produce occupancy pattern data of histone PTMs and histone variants, with higher resolution and higher signal to noise ratio. We further present an adaptation of this protocol to perform native ChIP from as low as 50,000 cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 23% |
| Researcher | 4 | 18% |
| Student > Postgraduate | 3 | 14% |
| Other | 2 | 9% |
| Student > Master | 2 | 9% |
| Other | 3 | 14% |
| Unknown | 3 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 41% |
| Biochemistry, Genetics and Molecular Biology | 6 | 27% |
| Immunology and Microbiology | 3 | 14% |
| Psychology | 1 | 5% |
| Unknown | 3 | 14% |