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DNA methylation regulates mouse cardiac myofibril gene expression during heart development

Overview of attention for article published in Journal of Biomedical Science, October 2015
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (75th percentile)
  • Good Attention Score compared to outputs of the same age and source (74th percentile)

Mentioned by

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8 tweeters

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7 Mendeley
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Title
DNA methylation regulates mouse cardiac myofibril gene expression during heart development
Published in
Journal of Biomedical Science, October 2015
DOI 10.1186/s12929-015-0203-6
Pubmed ID
Authors

Yang Xu, Lingjuan Liu, Bo Pan, Jing Zhu, Changlong Nan, Xupei Huang, Jie Tian

Abstract

It is well known that epigenetic modifications play an important role in controlling the regulation of gene expression during the development. Our previous studies have demonstrated that the expression of fetal troponin I gene (also called slow skeletal troponin I, ssTnI) is predominated in the fetal stage, reduced after birth and disappeared in the adulthood. The mechanism underlying the developmentally related ssTnI gene regulation is not clear. In this study, we have explored the epigenetic role of DNA methylation in the regulation of ssTnI expression in the heart during the development. The DNA methylation levels of CpG island and CpG dinucleotides region were detected using methylation specific PCR (MSP) and bisulfite sequence PCR (BSP) in 2000 bp upstream and 100 bp upstream of ssTnI gene promoter. Real time RT-PCR and Western blot were used to detect ssTnI mRNA and protein expression levels. We found that DNA methylation levels of the CpG dinucleotides region in ssTnI gene promoter were increased with the development, corresponding to a decreased expression of ssTnI gene in mouse heart. However the DNA methylation levels of CpG islands in this gene were not changed during the development. Application of a methylation inhibitor, 5-Azacytidine, in cultured myocardial cells partially prevented the decline of ssTnI expression. Our results indicate that DNA methylation, as an epigenetic intervention, plays a role in the regulation of the fetal TnI gene expression in the heat during the development.

Twitter Demographics

The data shown below were collected from the profiles of 8 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 14%
Unknown 6 86%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 29%
Student > Master 1 14%
Lecturer > Senior Lecturer 1 14%
Student > Ph. D. Student 1 14%
Librarian 1 14%
Other 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Computer Science 1 14%
Unspecified 1 14%
Agricultural and Biological Sciences 1 14%
Sports and Recreations 1 14%
Other 1 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 April 2016.
All research outputs
#1,859,504
of 8,616,558 outputs
Outputs from Journal of Biomedical Science
#57
of 383 outputs
Outputs of similar age
#60,795
of 249,200 outputs
Outputs of similar age from Journal of Biomedical Science
#7
of 27 outputs
Altmetric has tracked 8,616,558 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 383 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 249,200 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.