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An approach to the immunophenotypic features of circulating CD4+NKG2D+ T cells in invasive cervical carcinoma

Overview of attention for article published in Journal of Biomedical Science, October 2015
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  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (63rd percentile)

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3 tweeters

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22 Mendeley
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Title
An approach to the immunophenotypic features of circulating CD4+NKG2D+ T cells in invasive cervical carcinoma
Published in
Journal of Biomedical Science, October 2015
DOI 10.1186/s12929-015-0190-7
Pubmed ID
Authors

Mariel Garcia-Chagollan, Luis Felipe Jave-Suarez, Jesse Haramati, Miriam Ruth Bueno-Topete, Adriana Aguilar-Lemarroy, Ciro Estrada-Chavez, Blanca Estela Bastidas-Ramirez, Ana Laura Pereira-Suarez, Susana del Toro-Arreola

Abstract

NKG2D, an activating immunoreceptor, is primarily restricted to NK cells and CD8(+) T cells. The existence of an atypical cytotoxic CD4(+)NKG2D(+) T cell population has also been found in patients with autoimmune dysfunctions. Nonetheless, contradictory evidence has categorized this population with a regulatory rather than cytotoxic role in other situations. These confounding data have led to the proposal that two distinct CD4(+)NKG2D(+) T cell subsets might exist. The immune response elicited in cervical cancer has been characterized by apparent contradictions concerning the role that T cells, in particular T-helper cells, might be playing in the control of the tumor growth. Interestingly, we recently reported a substantial increase in the frequency of CD4(+)NKG2D(+) T cells in patients with cervical intraepithelial neoplasia grade-1. However, whether this particular population is also found in patients with more advanced cervical lesions or whether they express a distinctive phenotype remains still to be clarified. In this urgent study, we focused our attention on the immunophenotypic characterization of CD4(+)NKG2D(+) T cells in patients with well-established cervical carcinoma and revealed the existence of at least two separate CD4(+)NKG2D(+) T cell subsets defined by the co-expression or absence of CD28. Patients with diagnosis of invasive cervical carcinoma were enrolled in the study. A group of healthy individuals was also included. Multicolor flow cytometry was used for exploration of TCR alpha/beta, CD28, CD158b, CD45RO, HLA-DR, CD161, and CD107a. A Luminex-based cytokine kit was used to quantify the levels of pro- and anti-inflammatory cytokines. We found an increased percentage of CD4(+)NKG2D(+) T cells in patients with cervical cancer when compared with controls. Accordingly with an increase of CD4(+)NKG2D(+) T cells, we found decreased CD28 expression. The activating or degranulation markers HLA-DR, CD161, and CD107a were heterogeneously expressed. The levels of IL-1beta, IL-2, TNF-alpha, and IL-10 were negatively correlated with the percentages of CD4(+)NKG2D(+) T cells in patients with cervical carcinoma. Taken together, our results reveal the existence of two separate CD4(+)NKG2D(+) T cell subsets defined by the co-expression or absence of CD28, the latter more likely to be present in patients with cervical cancer.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 5%
Unknown 21 95%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 27%
Researcher 5 23%
Student > Ph. D. Student 4 18%
Student > Bachelor 1 5%
Student > Doctoral Student 1 5%
Other 2 9%
Unknown 3 14%
Readers by discipline Count As %
Medicine and Dentistry 6 27%
Biochemistry, Genetics and Molecular Biology 5 23%
Immunology and Microbiology 5 23%
Agricultural and Biological Sciences 2 9%
Unknown 4 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2016.
All research outputs
#3,689,681
of 7,926,939 outputs
Outputs from Journal of Biomedical Science
#173
of 354 outputs
Outputs of similar age
#104,066
of 241,860 outputs
Outputs of similar age from Journal of Biomedical Science
#8
of 22 outputs
Altmetric has tracked 7,926,939 research outputs across all sources so far. This one has received more attention than most of these and is in the 51st percentile.
So far Altmetric has tracked 354 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 241,860 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.