↓ Skip to main content

Protein Arginylation

Overview of attention for book
Cover of 'Protein Arginylation'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Protein Arginylation: Over 50 Years of Discovery.
  3. Altmetric Badge
    Chapter 2 Recollection of How We Came Across the Protein Modification with Amino Acids by Aminoacyl tRNA-Protein Transferase.
  4. Altmetric Badge
    Chapter 3 Arginyltransferase: A Personal and Historical Perspective.
  5. Altmetric Badge
    Chapter 4 Arginylation in a Partially Purified Fraction of 150k × g Supernatants of Axoplasm and Injured Vertebrate Nerves.
  6. Altmetric Badge
    Chapter 5 Preparation of ATE1 Enzyme from Native Mammalian Tissues.
  7. Altmetric Badge
    Chapter 6 Protein Arginylation
  8. Altmetric Badge
    Chapter 7 Assaying the Posttranslational Arginylation of Proteins in Cultured Cells.
  9. Altmetric Badge
    Chapter 8 Assaying ATE1 Activity in Yeast by β-Gal Degradation.
  10. Altmetric Badge
    Chapter 9 Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays.
  11. Altmetric Badge
    Chapter 10 Assaying ATE1 Activity In Vitro.
  12. Altmetric Badge
    Chapter 11 High-Throughput Arginylation Assay in Microplate Format.
  13. Altmetric Badge
    Chapter 12 Assay of Arginyltransferase Activity by a Fluorescent HPLC Method.
  14. Altmetric Badge
    Chapter 13 Identification of Arginylated Proteins by Mass Spectrometry.
  15. Altmetric Badge
    Chapter 14 Analysis of Arginylated Peptides by Subtractive Edman Degradation.
  16. Altmetric Badge
    Chapter 15 Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles.
  17. Altmetric Badge
    Chapter 16 Applying Arginylation for Bottom-Up Proteomics.
  18. Altmetric Badge
    Chapter 17 Development of New Tools for the Studies of Protein Arginylation.
Attention for Chapter 11: High-Throughput Arginylation Assay in Microplate Format.
Altmetric Badge

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
2 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
High-Throughput Arginylation Assay in Microplate Format.
Chapter number 11
Book title
Protein Arginylation
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2935-1_11
Pubmed ID
Book ISBNs
978-1-4939-2934-4, 978-1-4939-2935-1
Authors

Sougata Saha, Junling Wang, Anna S. Kashina

Editors

Anna S. Kashina

Abstract

Here we describe the biochemical assay for ATE1-mediated arginylation in microplate format, which can be applied to high-throughput screens for identification of small-molecule inhibitors and activators of ATE1, high-volume analysis of ATE1 substrates, and other similar applications. Originally, we have applied this screen to a library of 3280 compounds and identified two compounds which specifically affect ATE1-regulated processes in vitro and in vivo. The assay is based on in vitro ATE1-mediated arginylation of beta-actin's N-terminal peptide, but it can also be applied using other ATE1 substrates.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 50%
Lecturer 1 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 50%
Agricultural and Biological Sciences 1 50%