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IL-8 analogue CXCL8 (3-72) K11R/G31P, modulates LPS-induced inflammation via AKT1-NF-kβ and ERK1/2-AP-1 pathways in THP-1 monocytes

Overview of attention for article published in Human Immunology, August 2018
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Title
IL-8 analogue CXCL8 (3-72) K11R/G31P, modulates LPS-induced inflammation via AKT1-NF-kβ and ERK1/2-AP-1 pathways in THP-1 monocytes
Published in
Human Immunology, August 2018
DOI 10.1016/j.humimm.2018.08.007
Pubmed ID
Authors

Williams Walana, Jing-Jing Wang, Iddrisu Baba Yabasin, Michael Ntim, Sylvanus Kampo, Mahmoud Al-Azab, Abdalkhalig Elkhider, Eugene Dogkotenge Kuugbee, Jya-wei Cheng, John R. Gordon, Fang Li

Abstract

IL-8 is elevated during inflammation, and it initiates cascade of down-stream reactions. Its antagonist, CXCL8 (3-72) K11R/G31P (G31P), represses inflammatory reactions via competitive binding to CXC chemokine family, preferentially G protein-couple receptors (GPCRs) CXCR1/2. This study reports the effect of G31P on the transcription profile of lipopolysaccharide (LPS) induced inflammation in THP-1 monocytes ex-vivo. LPS (1µg/ml) induced elevation of IL-8 was significantly reduced by G31P (20 µg/ml and 30µg/ml), with relatively increased inhibition of CXCR2 than CXCR1. Transcription of IL-1β, IL-6, and TNF-α were significantly inhibited, while IL-10 remained relatively unchanged. G31P treatment also had repressing effect on the inflammatory associated enzymes COX-2, MMP-2, and MMP-9. Significant restriction of c-Fos, and NF-kβ mRNA expression was observed, while that of c-Jun was marginally elevated. Conversely, SP-1 mRNA expression was seen to increase appreciably by G31P treatment. While the translation of pAKT, pERK1/2, and p65- NF-kβ were down-regulated by the G31P following THP-1 cells stimulation with LPS, reactive oxygen species (ROS) expression was on the positive trajectory. Collectively, the IL-8 analogue, G31P, modulates the inflammatory profile of LPS induced inflammation in THP-1 monocytes via AKT1-NF-kβ and ERK1/2-AP-1 pathways.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 40%
Student > Ph. D. Student 1 20%
Student > Doctoral Student 1 20%
Unspecified 1 20%
Readers by discipline Count As %
Unspecified 2 40%
Pharmacology, Toxicology and Pharmaceutical Science 1 20%
Agricultural and Biological Sciences 1 20%
Immunology and Microbiology 1 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 August 2018.
All research outputs
#10,781,962
of 12,159,148 outputs
Outputs from Human Immunology
#1,203
of 1,341 outputs
Outputs of similar age
#206,889
of 245,773 outputs
Outputs of similar age from Human Immunology
#11
of 13 outputs
Altmetric has tracked 12,159,148 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,341 research outputs from this source. They receive a mean Attention Score of 3.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 245,773 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.