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α-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells

Overview of attention for article published in Scientific Reports, August 2018
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (63rd percentile)
  • Good Attention Score compared to outputs of the same age and source (74th percentile)

Mentioned by

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5 tweeters
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1 research highlight platform

Citations

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7 Dimensions

Readers on

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26 Mendeley
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Title
α-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells
Published in
Scientific Reports, August 2018
DOI 10.1038/s41598-018-30887-8
Pubmed ID
Authors

M. Krstić Ristivojević, J. Grundström, T. A. T. Tran, D. Apostolovic, V. Radoi, M. Starkhammar, V. Vukojević, T. Ćirković Veličković, C. Hamsten, M. van Hage

Abstract

Red meat allergy is characterized by an IgE response against the carbohydrate galactose-α-1,3-galactose (α-Gal), which is abundantly expressed on glycoproteins from non-primate mammals. The mechanisms of how α-Gal is processed and presented to the immune system to initiate an allergic reaction are still unknown. The aim of this study was to reveal whether the presence of α-Gal epitopes on the protein surface influence antigen uptake and processing in immature monocyte-derived dendritic cells (iMDDCs). Immature MDDCs were prepared from healthy blood donors and red meat allergic patients. We found an increased internalization of α-Gal carrying proteins over time in iMDDCs by flow cytometric analysis, which was independent of the donor allergic status. The uptake of α-Gal carrying proteins was significantly higher than the uptake of non-α-Gal carrying proteins. Confocal microscopy revealed α-Gal carrying proteins scattered around the cytoplasm in most iMDDCs while detection of proteins not carrying α-Gal was negligible. Fluorescent detection of protein on SDS-PAGE showed that degradation of α-Gal carrying proteins was slower than degradation of non-α-Gal carrying proteins. Thus, the presence of α-Gal on the protein surface affects both uptake and degradation of the protein, and the results add new knowledge of α-Gal as a clinically relevant food allergen.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 23%
Student > Ph. D. Student 5 19%
Student > Master 3 12%
Professor > Associate Professor 2 8%
Professor 1 4%
Other 3 12%
Unknown 6 23%
Readers by discipline Count As %
Medicine and Dentistry 7 27%
Agricultural and Biological Sciences 4 15%
Immunology and Microbiology 2 8%
Biochemistry, Genetics and Molecular Biology 1 4%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Other 2 8%
Unknown 9 35%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 May 2019.
All research outputs
#4,944,081
of 16,594,163 outputs
Outputs from Scientific Reports
#31,889
of 88,376 outputs
Outputs of similar age
#102,181
of 280,828 outputs
Outputs of similar age from Scientific Reports
#13
of 51 outputs
Altmetric has tracked 16,594,163 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 88,376 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 16.3. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,828 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.
We're also able to compare this research output to 51 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.