Title |
α-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells
|
---|---|
Published in |
Scientific Reports, August 2018
|
DOI | 10.1038/s41598-018-30887-8 |
Pubmed ID | |
Authors |
M. Krstić Ristivojević, J. Grundström, T. A. T. Tran, D. Apostolovic, V. Radoi, M. Starkhammar, V. Vukojević, T. Ćirković Veličković, C. Hamsten, M. van Hage |
Abstract |
Red meat allergy is characterized by an IgE response against the carbohydrate galactose-α-1,3-galactose (α-Gal), which is abundantly expressed on glycoproteins from non-primate mammals. The mechanisms of how α-Gal is processed and presented to the immune system to initiate an allergic reaction are still unknown. The aim of this study was to reveal whether the presence of α-Gal epitopes on the protein surface influence antigen uptake and processing in immature monocyte-derived dendritic cells (iMDDCs). Immature MDDCs were prepared from healthy blood donors and red meat allergic patients. We found an increased internalization of α-Gal carrying proteins over time in iMDDCs by flow cytometric analysis, which was independent of the donor allergic status. The uptake of α-Gal carrying proteins was significantly higher than the uptake of non-α-Gal carrying proteins. Confocal microscopy revealed α-Gal carrying proteins scattered around the cytoplasm in most iMDDCs while detection of proteins not carrying α-Gal was negligible. Fluorescent detection of protein on SDS-PAGE showed that degradation of α-Gal carrying proteins was slower than degradation of non-α-Gal carrying proteins. Thus, the presence of α-Gal on the protein surface affects both uptake and degradation of the protein, and the results add new knowledge of α-Gal as a clinically relevant food allergen. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 20% |
Unknown | 4 | 80% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 60% |
Science communicators (journalists, bloggers, editors) | 2 | 40% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 34 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 26% |
Researcher | 5 | 15% |
Student > Master | 4 | 12% |
Other | 2 | 6% |
Professor > Associate Professor | 2 | 6% |
Other | 4 | 12% |
Unknown | 8 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 8 | 24% |
Agricultural and Biological Sciences | 5 | 15% |
Biochemistry, Genetics and Molecular Biology | 2 | 6% |
Chemistry | 2 | 6% |
Immunology and Microbiology | 2 | 6% |
Other | 3 | 9% |
Unknown | 12 | 35% |