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The LINC Complex

Overview of attention for book
Cover of 'The LINC Complex'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 EM Tomography of Meiotic LINC Complexes
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    Chapter 2 Recombinant Purification of the Periplasmic Portion of the LINC Complex
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    Chapter 3 Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis
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    Chapter 4 Analysis of Nesprin-2 Interaction with Its Binding Partners and Actin
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    Chapter 5 Interactions of Nesprin-4-Containing LINC Complexes in Outer Hair Cells Explored by BioID
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    Chapter 6 Using Nesprin Tension Sensors to Measure Force on the LINC Complex
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    Chapter 7 Analyzing Mechanotransduction Through the LINC Complex in Isolated Nuclei
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    Chapter 8 Direct Force Probe for Nuclear Mechanics
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    Chapter 9 Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex-Dependent Mechanisms of Homeostatic Nuclear Positioning
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    Chapter 10 Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments
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    Chapter 11 Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy
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    Chapter 12 Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super-Resolution Imaging
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    Chapter 13 Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans
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    Chapter 14 High-Resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development
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    Chapter 15 Computational Methods for Studying the Plant Nucleus
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    Chapter 16 Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System
  18. Altmetric Badge
    Chapter 17 Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components
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    Chapter 18 Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice
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    Chapter 19 An In Vitro System to Measure the Positioning, Stiffness, and Rupture of the Nucleus in Skeletal Muscle
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    Chapter 20 Functional Analysis of LINC Complexes in the Skin
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    Chapter 21 Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer
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    Chapter 22 Next-Generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins
Attention for Chapter 12: Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super-Resolution Imaging
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (68th percentile)

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Chapter title
Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super-Resolution Imaging
Chapter number 12
Book title
The LINC Complex
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8691-0_12
Pubmed ID
Book ISBNs
978-1-4939-8690-3, 978-1-4939-8691-0

Unruh, Jay R, Slaughter, Brian D, Jaspersen, Sue L, Jay R. Unruh, Brian D. Slaughter, Sue L. Jaspersen, Unruh, Jay R., Slaughter, Brian D., Jaspersen, Sue L.


The Saccharomyces cerevisiae and Schizosaccharomyces pombe genomes encode a single SUN domain-containing protein, Mps3 and Sad1, respectively. Both localize to the yeast centrosome (known as the spindle pole body, SPB) and are essential for bipolar spindle formation. In addition, Mps3 and Sad1 play roles in chromosome organization in both mitotic and meiotic cells that are independent of their SPB function. To dissect the function of Mps3 at the nuclear envelope (NE) and SPB, we employed cell imaging methods such as scanning fluorescence cross-correlation spectroscopy (SFCCS) and single particle averaging with structured illumination microscopy (SPA-SIM) to determine the strength, nature, and location of protein-protein interactions in vivo. We describe how these same techniques can also be used in fission yeast to analyze Sad1, providing evidence of their applicability to other NE proteins and systems.

Twitter Demographics

The data shown below were collected from the profiles of 10 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 50%
Student > Postgraduate 1 50%
Readers by discipline Count As %
Unspecified 1 50%
Medicine and Dentistry 1 50%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 April 2019.
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Altmetric has tracked 14,708,827 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,777 research outputs from this source. They receive a mean Attention Score of 2.5. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 273,884 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them