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Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.

Overview of attention for article published in Journal of Bacteriology, January 1995
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (91st percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

twitter
1 tweeter
patent
216 patents
wikipedia
1 Wikipedia page

Citations

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3636 Dimensions

Readers on

mendeley
2091 Mendeley
citeulike
8 CiteULike
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1 Connotea
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Title
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.
Published in
Journal of Bacteriology, January 1995
DOI 10.1128/jb.177.14.4121-4130.1995
Pubmed ID
Authors

L M Guzman, D Belin, M J Carson, J Beckwith

Abstract

We have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD promoter of the araBAD (arabinose) operon and the gene encoding the positive and negative regulator of this promoter, araC. Using the phoA gene and phoA fusions to monitor expression in these vectors, we show that the ratio of induction/repression can be 1,200-fold, compared with 50-fold for PTAC-based vectors. phoA expression can be modulated over a wide range of inducer (arabinose) concentrations and reduced to extremely low levels by the presence of glucose, which represses expression. Also, the kinetics of induction and repression are very rapid and significantly affected by the ara allele in the host strain. Thus, the use of this system which can be efficiently and rapidly turned on and off allows the study of important aspects of bacterial physiology in a very simple manner and without changes of temperature. We have exploited the tight regulation of the PBAD promoter to study the phenotypes of null mutations of essential genes and explored the use of pBAD vectors as an expression system.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 2,091 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 42 2%
United Kingdom 18 <1%
Germany 14 <1%
Canada 10 <1%
Switzerland 4 <1%
Belgium 4 <1%
Spain 3 <1%
Chile 3 <1%
Netherlands 2 <1%
Other 22 1%
Unknown 1969 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 633 30%
Researcher 411 20%
Student > Bachelor 280 13%
Student > Master 275 13%
Student > Doctoral Student 84 4%
Other 266 13%
Unknown 142 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 1029 49%
Biochemistry, Genetics and Molecular Biology 521 25%
Immunology and Microbiology 84 4%
Chemistry 81 4%
Engineering 79 4%
Other 125 6%
Unknown 172 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 September 2020.
All research outputs
#1,086,019
of 15,903,488 outputs
Outputs from Journal of Bacteriology
#134
of 12,831 outputs
Outputs of similar age
#25,708
of 287,310 outputs
Outputs of similar age from Journal of Bacteriology
#2
of 64 outputs
Altmetric has tracked 15,903,488 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 12,831 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 287,310 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.