Chapter title |
Functional characterization of nematode effectors in plants.
|
---|---|
Chapter number | 9 |
Book title |
Plant-Pathogen Interactions
|
Published in |
Methods in molecular biology, March 2014
|
DOI | 10.1007/978-1-62703-986-4_9 |
Pubmed ID | |
Book ISBNs |
978-1-62703-985-7, 978-1-62703-986-4
|
Authors |
Elling AA, Jones JT, Axel A. Elling, John T. Jones, Elling, Axel A., Jones, John T. |
Abstract |
Secreted effectors represent the molecular interface between the nematode and its host plant. Studies that aimed at deciphering molecular plant-nematode interactions are hampered by technical hurdles that prevent the generation of transgenic nematodes. However, RNA interference (RNAi) has proven to be a valuable tool to specifically knock-down nematode effector genes, both ex planta and in planta. Plant-mediated RNAi of nematode genes not only facilitates functional characterization of effectors but also lends itself to a novel control strategy against plant-parasitic nematodes. Here, we describe currently used methods to silence genes in plant-parasitic cyst and root-knot nematodes. |
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