Title |
Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis
|
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Published in |
BMC Plant Biology, August 2018
|
DOI | 10.1186/s12870-018-1387-1 |
Pubmed ID | |
Authors |
Haroon Butt, Muhammad Jamil, Jian You Wang, Salim Al-Babili, Magdy Mahfouz |
Abstract |
Precision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known for their role in inhibiting shoot branching (tillering). We used CRISPR/Cas9 in rice (Oryza sativa) for targeted disruption of CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7), which controls a key step in SL biosynthesis. The ccd7 mutants exhibited a striking increase in tillering, combined with a reduced height, which could be rescued by application of the synthetic SL analog GR24. Striga germination assays and liquid chromatography-mass spectrometry analysis showed that root exudates of ccd7 mutants were also SL deficient. Taken together, our results show the potential and feasibility of the use of the CRISPR/Cas9 system for targeted engineering of plant architecture and for elucidating the molecular underpinnings of architecture-related traits. |
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Country | Count | As % |
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India | 1 | 14% |
United States | 1 | 14% |
Canada | 1 | 14% |
Unknown | 4 | 57% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 57% |
Scientists | 3 | 43% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 125 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 24 | 19% |
Student > Ph. D. Student | 17 | 14% |
Student > Master | 16 | 13% |
Student > Bachelor | 13 | 10% |
Student > Doctoral Student | 8 | 6% |
Other | 23 | 18% |
Unknown | 24 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 56 | 45% |
Biochemistry, Genetics and Molecular Biology | 31 | 25% |
Engineering | 2 | 2% |
Business, Management and Accounting | 1 | <1% |
Nursing and Health Professions | 1 | <1% |
Other | 5 | 4% |
Unknown | 29 | 23% |