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Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study

Overview of attention for article published in Lancet Infectious Diseases, November 2015
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  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#1 of 2,859)
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

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Title
Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study
Published in
Lancet Infectious Diseases, November 2015
DOI 10.1016/s1473-3099(15)00424-7
Pubmed ID
Authors

Liu, Yi-Yun, Wang, Yang, Walsh, Timothy R, Yi, Ling-Xian, Zhang, Rong, Spencer, James, Doi, Yohei, Tian, Guobao, Dong, Baolei, Huang, Xianhui, Yu, Lin-Feng, Gu, Danxia, Ren, Hongwei, Chen, Xiaojie, Lv, Luchao, He, Dandan, Zhou, Hongwei, Liang, Zisen, Liu, Jian-Hua, Shen, Jianzhong, Yi-Yun Liu, Yang Wang, Timothy R Walsh, Ling-Xian Yi, Rong Zhang, James Spencer, Yohei Doi, Guobao Tian, Baolei Dong, Xianhui Huang, Lin-Feng Yu, Danxia Gu, Hongwei Ren, Xiaojie Chen, Luchao Lv, Dandan He, Hongwei Zhou, Zisen Liang, Jian-Hua Liu, Jianzhong Shen, Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X, Yu LF, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu JH, Shen J

Abstract

Until now, polymyxin resistance has involved chromosomal mutations but has never been reported via horizontal gene transfer. During a routine surveillance project on antimicrobial resistance in commensal Escherichia coli from food animals in China, a major increase of colistin resistance was observed. When an E coli strain, SHP45, possessing colistin resistance that could be transferred to another strain, was isolated from a pig, we conducted further analysis of possible plasmid-mediated polymyxin resistance. Herein, we report the emergence of the first plasmid-mediated polymyxin resistance mechanism, MCR-1, in Enterobacteriaceae. The mcr-1 gene in E coli strain SHP45 was identified by whole plasmid sequencing and subcloning. MCR-1 mechanistic studies were done with sequence comparisons, homology modelling, and electrospray ionisation mass spectrometry. The prevalence of mcr-1 was investigated in E coli and Klebsiella pneumoniae strains collected from five provinces between April, 2011, and November, 2014. The ability of MCR-1 to confer polymyxin resistance in vivo was examined in a murine thigh model. Polymyxin resistance was shown to be singularly due to the plasmid-mediated mcr-1 gene. The plasmid carrying mcr-1 was mobilised to an E coli recipient at a frequency of 10(-1) to 10(-3) cells per recipient cell by conjugation, and maintained in K pneumoniae and Pseudomonas aeruginosa. In an in-vivo model, production of MCR-1 negated the efficacy of colistin. MCR-1 is a member of the phosphoethanolamine transferase enzyme family, with expression in E coli resulting in the addition of phosphoethanolamine to lipid A. We observed mcr-1 carriage in E coli isolates collected from 78 (15%) of 523 samples of raw meat and 166 (21%) of 804 animals during 2011-14, and 16 (1%) of 1322 samples from inpatients with infection. The emergence of MCR-1 heralds the breach of the last group of antibiotics, polymyxins, by plasmid-mediated resistance. Although currently confined to China, MCR-1 is likely to emulate other global resistance mechanisms such as NDM-1. Our findings emphasise the urgent need for coordinated global action in the fight against pan-drug-resistant Gram-negative bacteria. Ministry of Science and Technology of China, National Natural Science Foundation of China.

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Mendeley readers

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Geographical breakdown

Country Count As %
United States 16 1%
United Kingdom 10 <1%
Brazil 9 <1%
Denmark 4 <1%
Canada 4 <1%
India 4 <1%
France 4 <1%
Ukraine 3 <1%
Germany 2 <1%
Other 24 2%
Unknown 1103 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 248 21%
Student > Bachelor 218 18%
Student > Master 214 18%
Researcher 181 15%
Student > Postgraduate 71 6%
Other 251 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 390 33%
Medicine and Dentistry 210 18%
Biochemistry, Genetics and Molecular Biology 183 15%
Immunology and Microbiology 102 9%
Unspecified 74 6%
Other 224 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 1869. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 December 2017.
All research outputs
#434
of 8,769,624 outputs
Outputs from Lancet Infectious Diseases
#1
of 2,859 outputs
Outputs of similar age
#15
of 300,465 outputs
Outputs of similar age from Lancet Infectious Diseases
#1
of 82 outputs
Altmetric has tracked 8,769,624 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,859 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 26.1. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 300,465 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 82 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.