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Isolation and genetic characterization of Toxoplasma gondii from free-ranging and captive birds and mammals in Pernambuco state, Brazil

Overview of attention for article published in Revista Brasileira de Parasitologia Veterinária, August 2018
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Title
Isolation and genetic characterization of Toxoplasma gondii from free-ranging and captive birds and mammals in Pernambuco state, Brazil
Published in
Revista Brasileira de Parasitologia Veterinária, August 2018
DOI 10.1590/s1984-296120180059
Pubmed ID
Authors

Marcio André Silva, Hilda Fátima Jesus Pena, Herbert Sousa Soares, Juliana Aizawa, Solange Oliveira, Bruna Farias Alves, Dênisson Silva Souza, Renata Pimentel Bandeira Melo, Solange Maria Gennari, Rinaldo Aparecido Mota, Jean Carlos Ramos Silva

Abstract

Recent genetic population studies on Toxoplasma gondii in Brazil have shown large genetic variability. The objective of the present study was to isolate and genotypically characterize T. gondii from free-ranging and captive wild mammals and birds in Pernambuco state, Brazil. Fragments of heart, brain, skeletal muscle and diaphragm tissue from 71 birds and 34 mammals, which were either free-ranging or captive, were collected. Samples from 32 of these animals were subjected to bioassays in mice. Samples from the remaining 73 animals underwent biomolecular diagnosis, using PCR technique, targeting a repetitive DNA fragment of 529 bp in T. gondii. A non-virulent isolate (TgButstBrPE1) was obtained from a free-ranging striated heron (Butorides striata) and, based on primary samples, seven animals were found to be positive. The primary samples and the isolate obtained were subjected to PCR-RFLP using the markers SAG1, 5'3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3. ToxoDB-RFLP genotype #13 from the striated heron isolate and Type BrIII genotype from a captive otter ( Lontra longicaudis) (PS-TgLonloBrPE1) were obtained. The present study describes the first isolation and genotypic characterization of T. gondii in free-ranging striated heron, and the first genotypic characterization of T. gondii in a captive otter.

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Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 24%
Student > Bachelor 4 16%
Researcher 4 16%
Student > Ph. D. Student 3 12%
Other 2 8%
Other 3 12%
Unknown 3 12%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 7 28%
Agricultural and Biological Sciences 6 24%
Earth and Planetary Sciences 2 8%
Biochemistry, Genetics and Molecular Biology 2 8%
Nursing and Health Professions 1 4%
Other 2 8%
Unknown 5 20%