| Chapter title |
Pathogen Gene Expression Profiling During Infection Using a Nanostring nCounter Platform.
|
|---|---|
| Chapter number | 3 |
| Book title |
Yeast Functional Genomics
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3079-1_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3078-4, 978-1-4939-3079-1
|
| Authors |
Xu, Wenjie, Solis, Norma V, Filler, Scott G, Mitchell, Aaron P, Solis, Norma V., Filler, Scott G., Mitchell, Aaron P., Wenjie Xu, Norma V. Solis, Scott G. Filler, Aaron P. Mitchell |
| Abstract |
NanoString nCounter is a recently developed platform that can make direct multiplexed measurement of gene expression using color-coded probe pairs (Geiss et al., Nat Biotechnol 26(3):317-325, 2008; Malkov et al., BMC Res Notes 2:80, 2009). We have found that this platform is uniquely suitable for quantification of pathogen gene expression during infection, where pathogen RNA comprises a tiny portion of total RNA isolated from the infected tissue. Here, we describe a protocol that we have successfully applied to a number of pathogens across multiple infection models, including both invasive and mucosal infection by Candida albicans, and lung infection by Aspergillus fumigatus and Cryptococcus neoformans. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
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|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
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|---|---|---|
| New Zealand | 1 | 5% |
| Unknown | 21 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 36% |
| Student > Ph. D. Student | 4 | 18% |
| Student > Doctoral Student | 1 | 5% |
| Student > Bachelor | 1 | 5% |
| Student > Master | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 4 | 18% |
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|---|---|---|
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| Agricultural and Biological Sciences | 4 | 18% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Immunology and Microbiology | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 5 | 23% |