Chapter title |
Phosphopeptide Enrichment by Covalent Chromatography After Solid Phase Derivatization of Protein Digests on Reversed Phase Supports.
|
---|---|
Chapter number | 3 |
Book title |
Phospho-Proteomics
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3049-4_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3048-7, 978-1-4939-3049-4
|
Authors |
Heinz Nika, Ruth Hogue Angeletti, David H. Hawke |
Editors |
Louise von Stechow |
Abstract |
The isolation of the phosphopeptide constituents from phosphoprotein digests is prerequisite to facilitate the mass spectrometric characterization of phosphorylation events. Here, we describe a chemical proteomics approach which combines solid phase derivatization of phosphoprotein digests with phosphopeptide enrichment by covalent chromatography. The use of the solid phase support for derivatization ensures for speed and completeness of reactions. The isolates proved highly suitable for mapping of the sites of phosphorylation by collisionally induced dissociation (CID). The method combines robustness with simplicity of operation using equipment available in biological laboratories, and may be readily extended to map the sites of O-glycosylation. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 11% |
Unknown | 8 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 2 | 22% |
Professor > Associate Professor | 2 | 22% |
Student > Ph. D. Student | 1 | 11% |
Researcher | 1 | 11% |
Student > Master | 1 | 11% |
Other | 0 | 0% |
Unknown | 2 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Agricultural and Biological Sciences | 1 | 11% |
Computer Science | 1 | 11% |
Sports and Recreations | 1 | 11% |
Medicine and Dentistry | 1 | 11% |
Other | 1 | 11% |
Unknown | 2 | 22% |