Chapter title |
Phosphoprotein Detection by High-Throughput Flow Cytometry.
|
---|---|
Chapter number | 19 |
Book title |
Phospho-Proteomics
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3049-4_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3048-7, 978-1-4939-3049-4
|
Authors |
Johannes Landskron, Kjetil Taskén |
Editors |
Louise von Stechow |
Abstract |
Phospho flow cytometry is a powerful technique for the detection of protein phosphorylation events that, like Western blotting, relies on phospho-epitope-specific antibodies. In contrast to the latter, however, multidimensional and directly quantifiable data is obtained at the single-cell level allowing separate analysis of small cell populations in complex cellular mixtures. Furthermore, up to 30 phospho-specific antibodies or antibodies identifying other posttranslational modifications in combination with cell surface markers can be analyzed in a single experiment. Utilizing a technique called fluorescent cell barcoding that enables combination of up to 64 samples into one tube for multiplex analysis and later data deconvolution, phospho flow cytometry is turned into a medium- to high-throughput technology. |
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