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Phospho-Proteomics

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Cover of 'Phospho-Proteomics'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Thiol-ene-Enabled Detection of Thiophosphorylation as a Labeling Strategy for Phosphoproteins.
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    Chapter 2 Phosphopeptide Detection with Biotin-Labeled Phos-tag.
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    Chapter 3 Phosphopeptide Enrichment by Covalent Chromatography After Solid Phase Derivatization of Protein Digests on Reversed Phase Supports.
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    Chapter 4 Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics.
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    Chapter 5 Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles.
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    Chapter 6 Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM).
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    Chapter 7 Enrichment Strategies in Phosphoproteomics.
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    Chapter 8 Phosphopeptide Enrichment by Immobilized Metal Affinity Chromatography.
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    Chapter 9 The Use of Titanium Dioxide for Selective Enrichment of Phosphorylated Peptides.
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    Chapter 10 Sequential Elution from IMAC (SIMAC): An Efficient Method for Enrichment and Separation of Mono- and Multi-phosphorylated Peptides.
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    Chapter 11 Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation.
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    Chapter 12 Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome Coverage.
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    Chapter 13 Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview.
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    Chapter 14 Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics.
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    Chapter 15 Variable Digestion Strategies for Phosphoproteomics Analysis.
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    Chapter 16 Online LC-FAIMS-MS/MS for the Analysis of Phosphorylation in Proteins.
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    Chapter 17 Simple and Reproducible Sample Preparation for Single-Shot Phosphoproteomics with High Sensitivity.
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    Chapter 18 Identification of Direct Kinase Substrates via Kinase Assay-Linked Phosphoproteomics.
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    Chapter 19 Phosphoprotein Detection by High-Throughput Flow Cytometry.
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    Chapter 20 Resources for Assignment of Phosphorylation Sites on Peptides and Proteins.
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    Chapter 21 From Phosphosites to Kinases.
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    Chapter 22 Phospho-Proteomics
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    Chapter 23 Systems Analysis for Interpretation of Phosphoproteomics Data.
Attention for Chapter 20: Resources for Assignment of Phosphorylation Sites on Peptides and Proteins.
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Chapter title
Resources for Assignment of Phosphorylation Sites on Peptides and Proteins.
Chapter number 20
Book title
Phospho-Proteomics
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3049-4_20
Pubmed ID
Book ISBNs
978-1-4939-3048-7, 978-1-4939-3049-4
Authors

Ravikumar, Vaishnavi, Macek, Boris, Mijakovic, Ivan, Vaishnavi Ravikumar, Boris Macek, Ivan Mijakovic

Editors

Louise von Stechow

Abstract

Reversible protein phosphorylation is a key regulatory posttranslational modification that plays a significant role in major cellular signaling processes. Phosphorylation events can be systematically identified, quantified, and localized on protein sequence using publicly available bioinformatic tools. Here we present the software tools commonly used by the phosphoproteomics community, discuss their underlying principles of operation, and provide a protocol for large-scale phosphoproteome data analysis using the MaxQuant software suite.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 8%
Unknown 12 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 23%
Professor > Associate Professor 2 15%
Unspecified 1 8%
Student > Doctoral Student 1 8%
Lecturer > Senior Lecturer 1 8%
Other 2 15%
Unknown 3 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 31%
Agricultural and Biological Sciences 3 23%
Computer Science 2 15%
Unspecified 1 8%
Unknown 3 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 November 2015.
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#20,296,405
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Outputs from Methods in molecular biology
#9,916
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#330,625
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Outputs of similar age from Methods in molecular biology
#1,053
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