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X-Chromosome Inactivation

Overview of attention for book
Cover of 'X-Chromosome Inactivation'

Table of Contents

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    Book Overview
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    Chapter 1 Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs
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    Chapter 2 Unbiased Genetic Screen to Identify Factors Involved in X-Chromosome Inactivation Using a Pooled Bar-Coded shRNA Library
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    Chapter 3 ChIRP-MS: RNA-Directed Proteomic Discovery
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    Chapter 4 Manipulation of Xist Imprinting in Mouse Preimplantation Embryos
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    Chapter 5 Somatic Cell Nuclear Transfer in Mice: Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status
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    Chapter 6 Live Imaging of Xist RNA
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    Chapter 7 Live Imaging of X-Chromosome Inactivation and Reactivation Kinetics
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    Chapter 8 Visualizing the Dynamics of Inactive X Chromosomes in Living Cells Using Antibody-Based Fluorescent Probes
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    Chapter 9 Visualization of Hidden Epitopes at the Inactive X Chromosome
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    Chapter 10 Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation
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    Chapter 11 Simultaneous RNA–DNA FISH in Mouse Preimplantation Embryos
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    Chapter 12 Combined Immunofluorescence, RNA FISH, and DNA FISH in Preimplantation Mouse Embryos
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    Chapter 13 RNA-FISH and Immunofluorescence of Mouse Preimplantation and Postimplantation Embryos
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    Chapter 14 Experimental Analysis of Imprinted Mouse X-Chromosome Inactivation
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    Chapter 15 X-Chromosome Inactivation and Escape from X Inactivation in Mouse
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    Chapter 16 Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles
Attention for Chapter 3: ChIRP-MS: RNA-Directed Proteomic Discovery
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Chapter title
ChIRP-MS: RNA-Directed Proteomic Discovery
Chapter number 3
Book title
X-Chromosome Inactivation
Published in
Methods in molecular biology, September 2018
DOI 10.1007/978-1-4939-8766-5_3
Pubmed ID
Book ISBNs
978-1-4939-8765-8, 978-1-4939-8766-5
Authors

Ci Chu, Howard Y. Chang, Chu, Ci, Chang, Howard Y.

Abstract

Comprehensive identification of RNA-binding proteins by mass spectrometry (ChIRP-ms) is a novel technique for studying endogenous ribonucleoprotein complexes. ChIRP-ms is robust across a wide range of expression level, from abundant housekeeping RNAs (e.g., spliceosomal U RNAs) to relatively lowly expressed RNAs (e.g., Xist). In vivo RNA-protein interactions are chemically cross-linked, and purified using biotinylated antisense oligonucleotides against RNA of interest. Coprecipitated proteins are gently eluted, and identified by mass-spectrometry (for discovery) or by western blotting (for validation).

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 78 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 78 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 21%
Researcher 11 14%
Student > Bachelor 8 10%
Student > Master 7 9%
Student > Doctoral Student 5 6%
Other 9 12%
Unknown 22 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 26 33%
Agricultural and Biological Sciences 12 15%
Immunology and Microbiology 6 8%
Medicine and Dentistry 2 3%
Engineering 2 3%
Other 6 8%
Unknown 24 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 September 2018.
All research outputs
#20,533,292
of 23,103,436 outputs
Outputs from Methods in molecular biology
#9,978
of 13,208 outputs
Outputs of similar age
#294,089
of 337,900 outputs
Outputs of similar age from Methods in molecular biology
#188
of 248 outputs
Altmetric has tracked 23,103,436 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,208 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 337,900 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 248 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.